Effect of vitamin D 3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid.

Autor: Gezer A; Vocational School of Health Services, Ataturk University, Erzurum, Turkiye., Aras SY; Department of Midwifery, Faculty of Health Sciences, Kafkas University, Kars, Turkiye., Baygutalp NK; Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkiye., Sari EK; Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkiye., Bedir G; Department of Histology and Embryology, Ataturk University School of Medicine, Erzurum, Turkiye., Mokhtare B; Department of Pathology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkiye., Yilmaz K; Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkiye.
Jazyk: angličtina
Zdroj: Veterinarni medicina [Vet Med (Praha)] 2024 Mar 28; Vol. 69 (3), pp. 84-93. Date of Electronic Publication: 2024 Mar 28 (Print Publication: 2024).
DOI: 10.17221/111/2023-VETMED
Abstrakt: In this study, the effects of vitamin D 3 (Vit. D) and a stinging nettle [ Urtica dioica  L. (UD)] extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of Crohn's disease (CD). The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin (SSTR), aquaporin-1 (AQP-1), caspase-3, and tumour necrosis factor-alpha (TNF-α) immunoreactivity in the gastric tissues. In the evaluations, degenerative and necrotic changes and mononuclear cell infiltration areas were observed in the TNBS group, but such changes could be improved with Vit. D and UD applications. The results suggest that the combination of the Vit. D and UD extract may have a protective and therapeutic role in mitigating TNBS-induced damage to the gastric tissues, potentially through the regulation of SSTR, AQP-1, caspase-3, and TNF-α expression. This indicates a promising avenue for further research and the exploration of these compounds in the context of gastrointestinal health.
Competing Interests: The authors declare no conflict of interest.
(Copyright: © 2024 Gezer et al.)
Databáze: MEDLINE
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