DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity.

Autor: Prostova M; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. prostovna@gmail.com., Kanevskaya A; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. annakanevskaja@yandex.ru., Panteleev V; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Lisitskaya L; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Perfilova Tugaeva KV; A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Sluchanko NN; A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Esyunina D; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Kulbachinskiy A; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. avkulb@yandex.ru.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 May; Vol. 9 (5), pp. 1368-1381. Date of Electronic Publication: 2024 Apr 15.
DOI: 10.1038/s41564-024-01654-5
Abstrakt: Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR-Cas, detect and cleave invader nucleic acids using complementary guides and the nuclease activities of pAgo or Cas proteins. However, not all pAgos are active nucleases. A large clade of short pAgos bind nucleic acid guides but lack nuclease activity, suggesting a different mechanism of action. Here we investigate short pAgos associated with a putative effector nuclease, NbaAgo from Novosphingopyxis baekryungensis and CmeAgo from Cupriavidus metallidurans. We show that these pAgos form a heterodimeric complex with co-encoded effector nucleases (short prokaryotic Argonaute, DNase and RNase associated (SPARDA)). RNA-guided target DNA recognition unleashes the nuclease activity of SPARDA leading to indiscriminate collateral cleavage of DNA and RNA. Activation of SPARDA by plasmids or phages results in degradation of cellular DNA and cell death or dormancy, conferring target-specific population protection and expanding the range of known prokaryotic immune systems.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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