Age-Dependent Changes in the Production of Mitochondrial Reactive Oxygen Species in Human Skeletal Muscle.

Autor: Vyssokikh MY; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia. mikhail.vyssokikh@gmail.com.; National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V. I. Kulakov, Moscow, 117997, Russia.; Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia., Vigovskiy MA; Medical Research and Education Center, Lomonosov Moscow State University, Moscow, 119192, Russia., Philippov VV; Medical Research and Education Center, Lomonosov Moscow State University, Moscow, 119192, Russia., Boroday YR; Medical Research and Education Center, Lomonosov Moscow State University, Moscow, 119192, Russia., Marey MV; National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V. I. Kulakov, Moscow, 117997, Russia., Grigorieva OA; Medical Research and Education Center, Lomonosov Moscow State University, Moscow, 119192, Russia., Vepkhvadze TF; Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia., Kurochkina NS; Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia., Manukhova LA; National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V. I. Kulakov, Moscow, 117997, Russia., Efimenko AY; Medical Research and Education Center, Lomonosov Moscow State University, Moscow, 119192, Russia., Popov DV; Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia., Skulachev VP; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
Jazyk: angličtina
Zdroj: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2024 Feb; Vol. 89 (2), pp. 299-312.
DOI: 10.1134/S0006297924020093
Abstrakt: A decrease in muscle mass and its functionality (strength, endurance, and insulin sensitivity) is one of the integral signs of aging. One of the triggers of aging is an increase in the production of mitochondrial reactive oxygen species. Our study was the first to examine age-dependent changes in the production of mitochondrial reactive oxygen species related to a decrease in the proportion of mitochondria-associated hexokinase-2 in human skeletal muscle. For this purpose, a biopsy was taken from m. vastus lateralis in 10 young healthy volunteers and 70 patients (26-85 years old) with long-term primary arthrosis of the knee/hip joint. It turned out that aging (comparing different groups of patients), in contrast to inactivity/chronic inflammation (comparing young healthy people and young patients), causes a pronounced increase in peroxide production by isolated mitochondria. This correlated with the age-dependent distribution of hexokinase-2 between mitochondrial and cytosolic fractions, a decrease in the rate of coupled respiration of isolated mitochondria and respiration when stimulated with glucose (a hexokinase substrate). It is discussed that these changes may be caused by an age-dependent decrease in the content of cardiolipin, a potential regulator of the mitochondrial microcompartment containing hexokinase. The results obtained contribute to a deeper understanding of age-related pathogenetic processes in skeletal muscles and open prospects for the search for pharmacological/physiological approaches to the correction of these pathologies.
Databáze: MEDLINE
Externí odkaz: