| Autor: |
Ramirez-Calderon G; Laboratory of Stem Cells and Diseases, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia., Saleh A; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia., Hidalgo Castillo TC; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia., Druet V; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia., Almarhoon B; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia., Almulla L; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia., Adamo A; Laboratory of Stem Cells and Diseases, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia., Inal S; Organic Bioelectronics Laboratory, Biological and Environmental Science and Engineering Division, KAUST, Thuwal 23955-6900, Saudi Arabia. |
| Abstrakt: |
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are a promising cell source for cardiac regenerative medicine and in vitro modeling. However, hPSC-CMs exhibit immature structural and functional properties compared with adult cardiomyocytes. Various electrical, mechanical, and biochemical cues have been applied to enhance hPSC-CM maturation but with limited success. In this work, we investigated the potential application of the semiconducting polymer poly{[N,N'-bis(2-octyldodecyl)-naphthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]-alt-5,5'-(2,2'-bithiophene)} (P(NDI2OD-T2)) as a light-sensitive material to stimulate hPSC-CMs optically. Our results indicated that P(NDI2OD-T2)-mediated photostimulation caused cell damage at irradiances applied long-term above 36 μW/mm 2 and did not regulate cardiac monolayer beating (after maturation) at higher intensities applied in a transient fashion. However, we discovered that the cells grown on P(NDI2OD-T2)-coated substrates showed significantly enhanced expression of cardiomyocyte maturation markers in the absence of a light exposure stimulus. A combination of techniques, such as atomic force microscopy, scanning electron microscopy, and quartz crystal microbalance with dissipation monitoring, which we applied to investigate the interface of the cell with the n-type coating, revealed that P(NDI2OD-T2) impacted the nanostructure, adsorption, and viscoelasticity of the Matrigel coating used as a cell adhesion promoter matrix. This modified cellular microenvironment promoted the expression of cardiomyocyte maturation markers related to contraction, calcium handling, metabolism, and conduction. Overall, our findings demonstrate that conjugated polymers such as P(NDI2OD-T2) can be used as passive coatings to direct stem cell fate through interfacial engineering of cell growth substrates. |
