Application of Single Cell Gene Expression Technologies to Neurotoxicology.

Autor: Tukker AM; School of Health Sciences, Purdue University, West Lafayette, IN, USA., Bowman AB; School of Health Sciences, Purdue University, West Lafayette, IN, USA.
Jazyk: angličtina
Zdroj: Current opinion in toxicology [Curr Opin Toxicol] 2024 Mar; Vol. 37. Date of Electronic Publication: 2023 Dec 27.
DOI: 10.1016/j.cotox.2023.100458
Abstrakt: Neurotoxicological research faces the challenge of linking biological changes resulting from exposures to neuronal function. An additional challenge is understanding cell-type specific differences and selective vulnerabilities of distinct neuronal populations to toxic insults. Single cell RNA-sequencing (scRNA-seq) allows for measurement of the transcriptome of individual cells. This makes it a valuable tool for validating and characterizing cell types present in multicell type samples in complex tissue or cell culture models, but also for understanding how different cell types respond to toxic insults. Pathway analysis of differentially expressed genes can provide in depth insights into underlying cell type-specific mechanisms of neurotoxicity. Toxicological data often has to be translated to outcomes for human health which requires an understanding of inter-species differences. Transcriptomic data aids in understanding these differences, including understanding developmental timelines of different species. We believe that scRNA-seq holds exciting promises for future neurotoxicological research.
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze: MEDLINE
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