Interindividual differences of dietary fat-inducible Mest in white adipose tissue of C57BL/6J mice are not heritable.

Autor: Anunciado-Koza RVP; MaineHealth Institute for Research, Scarborough, Maine, USA., Yin H; MaineHealth Institute for Research, Scarborough, Maine, USA., Bilodeau CL; MaineHealth Institute for Research, Scarborough, Maine, USA., Cooke D; Orentreich Foundation for the Advancement of Science, Inc., Cold Spring, New York, USA., Ables GP; Orentreich Foundation for the Advancement of Science, Inc., Cold Spring, New York, USA., Ryzhov S; MaineHealth Institute for Research, Scarborough, Maine, USA.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.; Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts, USA., Koza RA; MaineHealth Institute for Research, Scarborough, Maine, USA.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.; Pennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
Jazyk: angličtina
Zdroj: Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2024 Jun; Vol. 32 (6), pp. 1144-1155. Date of Electronic Publication: 2024 Apr 14.
DOI: 10.1002/oby.24020
Abstrakt: Objective: Differences in white adipose tissue (WAT) expression of mesoderm-specific transcript (Mest) in C57BL6/J mice fed a high-fat diet (HFD) are concomitant with and predictive for the development of obesity. However, the basis for differences in WAT Mest among mice is unknown. This study investigated whether HFD-inducible WAT Mest, as well as susceptibility to obesity, is transmissible from parents to offspring.
Methods: WAT biopsies of mice fed an HFD for 2 weeks identified parents with low and high WAT Mest for breeding. Obesity phenotypes, WAT Mest, hepatic gene expression, and serum metabolites were determined in offspring fed an HFD for 2 weeks.
Results: Offspring showed no heritability of obesity or WAT Mest phenotypes from parents but did show hepatic and serum metabolite changes consistent with their WAT Mest. Importantly, retired male breeders showed WAT Mest expression congruent with initial WAT biopsies even though HFD exposure occurred early in life.
Conclusions: Disparity of HFD-induced Mest in mice is not heritable but, rather, is reestablished during each generation and remains fixed from an early age to adulthood. Short-term HFD feeding reveals variation of WAT Mest expression within isogenic mice that is positively associated with the development of obesity.
(© 2024 The Obesity Society.)
Databáze: MEDLINE
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