Antimicrobial resistance in rural rivers: Comparative study of the Coquet (Northumberland) and Eden (Cumbria) River catchments.

Autor: Robins K; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., O'Donnell G; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Neumann A; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Schmidt W; Chief Scientists Group, Environment Agency, Horizon House, Deanery Road, Bristol BS1 5AH, UK., Hart A; Chief Scientists Group, Environment Agency, Horizon House, Deanery Road, Bristol BS1 5AH, UK., Graham DW; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, UK. Electronic address: david.graham@newcastle.ac.uk.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Jun 10; Vol. 928, pp. 172348. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1016/j.scitotenv.2024.172348
Abstrakt: Many studies have characterised resistomes in river microbial communities. However, few have compared resistomes in parallel rural catchments that have few point-source inputs of antimicrobial genes (ARGs) and organisms (i.e., AMR) - catchments where one can contrast more nebulous drivers of AMR in rural rivers. Here, we used quantitative microbial profiling (QMP) to compare resistomes and microbiomes in two rural river catchments in Northern England, the Coquet and Eden in Northumberland and Cumbria, respectively, with different hydrological and geographical conditions. The Eden has higher flow rates, higher annual surface runoff, and longer periods of soil saturation, whereas the Coquet is drier and has lower flowrates. QMP analysis showed the Eden contained significantly more abundant microbes associated with soil sources, animal faeces, and wastewater than the Coquet, which had microbiomes like less polluted rivers (Wilcoxon test, p < 0.01). The Eden also had greater ARG abundances and resistome diversity (Kruskal Wallis, p < 0.05), and higher levels of potentially clinically relevant ARGs. The Eden catchment had greater and flashier runoff and more extensive agricultural land use in its middle reach, which explains higher levels of AMR in the river. Hydrological and geographic factors drive AMR in rural rivers, which must be considered in environmental monitoring programmes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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