Influence of diimine bidentate ligand in the nitrosyl and nitro terpyridine ruthenium complex on the HSA/DNA interaction and antiviral activity.

Autor: Bessas NC; Instituto de Química, Universidade Federal de Uberlândia, Avenida João Naves de Avila, 2121, 38400-902, Uberlândia, MG, Brazil., Christine de Souza Arantes E; Instituto de Química, Universidade Federal de Uberlândia, Avenida João Naves de Avila, 2121, 38400-902, Uberlândia, MG, Brazil., Cassani NM; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Aquino Ruiz UE; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Santos IA; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Silva Martins DO; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil; Instituto de Ciências Exatas e Naturais Do Pontal, ICENP, Universidade Federal de Uberlândia, Rua Vinte, 1600, 38304-402, Tupã, Ituiutaba, MG, Brazil., Costa Oliveira AL; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Antoniucci GA; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., de Oliveira AHC; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, CEP 14040-901, Ribeirão Preto, SP, Brazil., DeFreitas-Silva G; Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, 31.270-901, Belo Horizonte, MG, Brazil., Gomes Jardim AC; Laboratory of Antiviral Research, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil., Galvão de Lima R; Instituto de Química, Universidade Federal de Uberlândia, Avenida João Naves de Avila, 2121, 38400-902, Uberlândia, MG, Brazil; Instituto de Ciências Exatas e Naturais Do Pontal, ICENP, Universidade Federal de Uberlândia, Rua Vinte, 1600, 38304-402, Tupã, Ituiutaba, MG, Brazil. Electronic address: renatagalvao@ufu.br.
Jazyk: angličtina
Zdroj: Nitric oxide : biology and chemistry [Nitric Oxide] 2024 Jun 01; Vol. 147, pp. 26-41. Date of Electronic Publication: 2024 Apr 11.
DOI: 10.1016/j.niox.2024.04.006
Abstrakt: Nitric oxide (NO) acts in different physiological processes, such as blood pressure control, antiparasitic activities, neurotransmission, and antitumor action. Among the exogenous NO donors, ruthenium nitrosyl/nitro complexes are potential candidates for prodrugs, due to their physicochemical properties, such as thermal and physiological pH stability. In this work, we proposed the synthesis and physical characterization of the new nitro terpyridine ruthenium (II) complexes of the type [Ru II (L)(NO 2 )(tpy)]PF 6 where tpy = 2,2':6',2″-terpyridine; L = 3,4-diaminobenzoic acid (bdq) or o-phenylenediamine (bd) and evaluation of influence of diimine bidentate ligand NH.NHq-R (R = H or COOH) in the HSA/DNA interaction as well as antiviral activity. The interactions between HSA and new nitro complexes [Ru II (L)(NO 2 )(tpy)] + were evaluated. The K a values for the HSA-[Ru II (bdq)(NO 2 )(tpy)] + is 10 times bigger than HSA-[Ru II (bd)(NO 2 )(tpy)] + . The sites of interaction between HSA and the complexes via synchronous fluorescence suppression indicate that the [Ru II (bdq)(NO 2 )(tpy)] + is found close to the Trp-241 residue, while the [Ru II (bd)(NO 2 )(tpy)] + complex is close to Tyr residues. The interaction with fish sperm fs-DNA using direct spectrophotometric titration (K b ) and ethidium bromide replacement (K SV and K app ) showed weak interaction in the system fs-DNA-[Ru II (bdq)(NO)(tpy)] + . Furthermore, fs-DNA-[Ru II (bd)(NO 2 )(tpy)] + and fs-DNA-[Ru II (bd)(NO)(tpy)] 3+ system showed higher intercalation constant. Circular dichroism spectra for fs-DNA-[Ru II (bd)(NO 2 )(tpy)] + and fs-DNA-[Ru II (bd)(NO)(tpy)] 3+ , suggest semi-intercalative accompanied by major groove binding interaction modes. The [Ru II (bd)(NO 2 )(tpy)] + and [Ru II (bd)(NO)(tpy)] 3+ inhibit replication of Zika and Chikungunya viruses based in the nitric oxide release under S-nitrosylation reaction with cysteine viral.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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