| Autor: |
Parra M; Laboratory of Natural Products Chemistry, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile.; Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Aldabaldetrecu M; Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Arce P; Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Soto-Aguilera S; Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Vargas R; Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile.; Aquaculture Production Unit, Universidad of Los Lagos, Osorno 5290000, Chile., Guerrero J; Laboratory of Coordination Compounds and Supramolecularity, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Tello M; Laboratory of Bacterial Metagenomic, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile., Modak B; Laboratory of Natural Products Chemistry, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9160000, Chile. |
| Abstrakt: |
Piscirickettsia salmonis is the pathogen that most affects the salmon industry in Chile. Large quantities of antibiotics have been used to control it. In search of alternatives, we have developed [Cu(NN 1 ) 2 ]ClO 4 where NN 1 = 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one. The antibacterial capacity of [Cu(NN 1 ) 2 ]ClO 4 was determined. Subsequently, the effect of the administration of [Cu(NN 1 ) 2 ]ClO 4 on the growth of S. salar , modulation of the immune system and the intestinal microbiota was studied. Finally, the ability to protect against a challenge with P. salmonis was evaluated. The results obtained showed that the compound has an MIC between 15 and 33.9 μg/mL in four isolates. On the other hand, the compound did not affect the growth of the fish; however, an increase in the transcript levels of IFN-γ, IL-12, IL-1β, CD4, lysozyme and perforin was observed in fish treated with 40 μg/g of fish. Furthermore, modulation of the intestinal microbiota was observed, increasing the genera of beneficial bacteria such as Lactobacillus and Bacillus as well as potential pathogens such as Vibrio and Piscirickettsia . Finally, the treatment increased survival in fish challenged with P. salmonis by more than 60%. These results demonstrate that the compound is capable of protecting fish against P. salmonis , probably by modulating the immune system and the composition of the intestinal microbiota. |
