| Autor: |
Santosaningsih D; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang 65142, Indonesia.; Department of Clinical Microbiology, Dr. Saiful Anwar Hospital, Malang 65112, Indonesia., Mulyastuti Y; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang 65142, Indonesia., Poejiani S; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang 65142, Indonesia., Putri RF; Magister of Chemistry Study Program, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia., Dewi L; School of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang 65142, Indonesia., Arifani H; School of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang 65142, Indonesia., Ni'mah YL; Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia., Baktir A; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia. |
| Abstrakt: |
Biofilm-related infections play a significant role in the development and persistence of ventilator-associated pneumonia. Pseudomonas aeruginosa ( P. aeruginosa ) frequently causes biofilm-related infections associated with ventilator tubing. Glucosamine gold nanoparticles (AuNPs) may exhibit antibiofilm properties; however, more studies, including combinatorial therapy with antibiotics, are needed to explore their potential applications in clinical settings. This study aims to investigate the biofilm inhibition properties of glucosamine AuNPs in combination with meropenem against P. aeruginosa ATCC 9027 on the endotracheal tube. A biofilm inhibition assay of glucosamine AuNPs at 0.02 mg/mL, both singly and in combination with meropenem at 1 mg/mL, was carried out against P. aeruginosa ATCC 9027 on an endotracheal tube using the tissue culture plate method. Scanning electron microscopy was performed for visualization. Glucosamine AuNPs at 0.02 mg/mL combined with meropenem at 1 mg/mL showed greater biofilm inhibition (72%) on the endotracheal tube than glucosamine nanoparticles at 0.02 mg/mL alone (26%) ( p = 0.001). The scanning electron microscopic visualization revealed that the untreated P. aeruginosa biofilm was denser than the glucosamine nanoparticles-treated biofilm, whether combined with meropenem or using glucosamine nanoparticles alone. The combination of glucosamine AuNPs and meropenem may have the synergistic effect of inhibiting biofilm production of P. aeruginosa on the endotracheal tubes of patients with mechanical ventilation. Conducting additional experiments to explore the impact of combining glucosamine-coated gold nanoparticles (AuNPs) with meropenem on the inhibition of biofilm production by clinical P. aeruginosa isolates would be beneficial. |
