Human neural stem cell secretome relieves endoplasmic reticulum stress-induced apoptosis and improves neuronal functions after traumatic brain injury in a rat model.

Autor: Ling Y; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; Department of Laboratory Medicine, Nanjing Red Cross Blood Center, Nanjing, 210003, Jiangsu, China., Ramalingam M; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, 31116, Republic of Korea.; School of Basic Medical Sciences, Chengdu University, Chengdu, 610106, China., Lv X; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Niu D; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Zeng Y; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Qiu Y; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Si Y; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Guo T; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Ni Y; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Zhang J; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China., Wang Z; Health Clinical Laboratories, Health BioMed Co., Ltd. Ningbo, Zhejiang, 315042, China., Kim HW; Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, 31116, Republic of Korea.; School of Basic Medical Sciences, Chengdu University, Chengdu, 610106, China., Hu J; Institute of Cerebrovascular Disease, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China. hu@ujs.edu.cn.; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. hu@ujs.edu.cn.
Jazyk: angličtina
Zdroj: Journal of molecular histology [J Mol Histol] 2024 Jun; Vol. 55 (3), pp. 329-348. Date of Electronic Publication: 2024 Apr 12.
DOI: 10.1007/s10735-024-10192-7
Abstrakt: Neural stem cell secretome (NSC-S) plays an important role in neuroprotection and recovery. Studies have shown that endoplasmic reticulum stress (ER stress) is involved in the progression of traumatic brain injury (TBI) and is a crucial cause of secondary damage and neuronal death after brain injury. Whether NSC-S is engaged in ER stress and ER stress-mediated neuronal apoptosis post-TBI has not been investigated. In the study, the Feeney SD male rat model was established. The results showed that NSC-S treatment significantly improved the behavior of rats with TBI. In addition, NSC-S relieved ER stress in TBI rats and was observed by transmission electron microscopy and western blot. The specific mechanism was further elucidated that restoration was achieved by alleviating the PERK-eIF2α pathway and thus protecting neurons from apoptosis. Notably, the discovery of calumenin (CALU) in NSC-S by liquid chromatography-tandem mass spectrometry (LC-MS/MS/MS) may be related to the protective effect of NSC-S on ER stress in neurons. Also, the mechanism by which it functions may be related to ubiquitination. In summary, NSC-S improved prognosis and ER stress in TBI rats and might be a promising treatment for relieving TBI.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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