Evaluation of sponge wipe surface sampling for collection of potential surrogates for non-spore-forming bioterrorism agents.

Autor:	Aslett LD; Jacobs Technology, Inc., 600 William Northern Blvd, Tullahoma, TN 37388, United States., Calfee MW; Office of Research and Development, US EPA, Research Triangle Park, NC 27711, United States., Monge M; CSS, Inc., 10301 Democracy Lane, Suite 300, Fairfax, VA 22030, United States., Abdel-Hady A; Jacobs Technology, Inc., 600 William Northern Blvd, Tullahoma, TN 37388, United States., Chamberlain T; Jacobs Technology, Inc., 600 William Northern Blvd, Tullahoma, TN 37388, United States., Baartmans R; Intellectechs, Inc., 195 S. Rosemont Road, Suite 103, VA Beach, VA 23452, United States., Touati A; Jacobs Technology, Inc., 600 William Northern Blvd, Tullahoma, TN 37388, United States.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 May 01; Vol. 135 (5).
DOI:	10.1093/jambio/lxae097
Abstrakt:	Aim: Evaluate the efficacy of sponge wipe sampling at recovering potential bacterial surrogates for Category A and B non-spore-forming bacterial bioterrorism agents from hard, nonporous surfaces. Methods: A literature survey identified seven nonpathogenic bacteria as potential surrogates for selected Category A and B non-spore-forming bacterial agents. Small (2 × 4 cm) and large (35.6 × 35.6 cm) coupons made from either stainless steel, plastic, or glass, were inoculated and utilized to assess persistence and surface sampling efficiency, respectively. Three commercially available premoistened sponge wipes (3M™, Sani-Stick®, and Solar-Cult®) were evaluated. Results: Mean recoveries from persistence testing indicated that three microorganisms (Yersinia ruckeri, Escherichia coli, and Serratia marcescens) demonstrated sufficient persistence across all tested material types. Sampling of large inoculated (≥107 CFU per sample) coupons resulted in mean recoveries ranging from 6.6 to 3.4 Log10 CFU per sample. Mean recoveries for the Solar-Cult®, 3M™ sponge wipes, and Sani-Sticks® across all test organisms and all material types were ≥5.7, ≥3.7, and ≥3.4 Log10 CFU per sample, respectively. Mean recoveries for glass, stainless steel, and ABS plastic across all test organisms and all sponge types were ≥3.8, ≥3.7, and ≥3.4 Log10 CFU per sample, respectively. Conclusions: Recovery results suggest that sponge wipe sampling can effectively be used to recover non-spore-forming bacterial cells from hard, nonporous surfaces such as stainless steel, ABS plastic, and glass. (Published by Oxford University Press on behalf of Applied Microbiology International 2024.)
Databáze:	MEDLINE
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