Peptide Extract from Red Kidney Beans, Phaseolus vulgaris (Fabaceae), Shows Promising Antimicrobial, Antibiofilm, and Quorum Sensing Inhibitory Effects.

Autor: Tetteh J; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Wereko Brobbey DY; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Osei KJ; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Ayamah A; Department of Crop and Soil Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Laryea MK; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Darko G; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Borquaye LS; Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.; Central Laboratory, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Jazyk: angličtina
Zdroj: Biochemistry research international [Biochem Res Int] 2024 Apr 04; Vol. 2024, pp. 4667379. Date of Electronic Publication: 2024 Apr 04 (Print Publication: 2024).
DOI: 10.1155/2024/4667379
Abstrakt: The rapid spread of multidrug-resistant bacteria has led to an increased risk of infectious diseases. Pseudomonas aeruginosa , in particular, poses a significant obstacle due to its propensity to rapidly acquire resistance to conventional antibiotics. This has resulted in an urgent need for the development of new classes of antibiotics that do not induce resistance. Antimicrobial peptides (AMPs) have been studied as potential small-molecule antibiotics due to their unique mode of action. In this study, peptides were extracted from the seeds of Phaseolus vulgaris (Fabaceae), and the antimicrobial activities of the extract were evaluated using microbroth dilution against five different microorganisms. The extract showed antimicrobial activity against all tested organisms with minimum inhibitory concentrations (MIC) of 2.5 mg/mL, except for Candida albicans and Pseudomonas aeruginosa , which had MICs of 1.25 mg/mL. The extract was also bacteriostatic for all tested organisms. The crude peptide extract from Phaseolus vulgaris was further studied for its antibiofilm activity against Pseudomonas aeruginosa , a common nosocomial pathogen associated with biofilm formation. The extract showed good antibiofilm activity at 1/2 MIC. The extract also inhibited the expression of pyocyanin and pyoverdine (virulence factors of P. aeruginosa whose expression is mediated by quorum sensing) by 82% and 66%, respectively. These results suggest that the peptide mix from Phaseolus vulgaris may inhibit biofilm formation and virulence factor expression by interfering with cell-to-cell communication in Pseudomonas aeruginosa . The ability of the extract to inhibit the growth and biofilm formation of all tested organisms indicates its potential as an antimicrobial agent that could be further studied for drug discovery.
Competing Interests: LSB, GD, and MKL are faculty at the Department of Chemistry, KNUST, where they lead a group of vibrant, young researchers in the Borquaye Research Group (https://www.borquayelab.com). JT, DWB, and KJO are members of the Borquaye Research Group. They hold B.Sc degrees in Chemistry from KNUST. AA is a technical staff at the Department of Crop and Soil Sciences, KNUST.
(Copyright © 2024 Jennifer Tetteh et al.)
Databáze: MEDLINE
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