Fibroblast matrix implants-a better alternative for incisional hernia repair?

Autor:	Hendrawan S; Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia.; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia., Lheman J; Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia., Weber U; Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia.; Baermed, Centre of Abdominal Surgery, Hirslanden Clinic, 8032 Zürich, Switzerland., Oberkofler CE; Vivévis AG, Viszeral-, Tumor- und Roboterchirurgie, Kappelistrasse 7, 8002 Zürich, Switzerland., Eryani A; Department of Histology, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia., Vonlanthen R; Vivévis AG, Viszeral-, Tumor- und Roboterchirurgie, Kappelistrasse 7, 8002 Zürich, Switzerland., Baer HU; Tarumanagara Human Cell Technology Laboratory, Faculty of Medicine, Tarumanagara University, Jakarta 11440, Indonesia.; Baermed, Centre of Abdominal Surgery, Hirslanden Clinic, 8032 Zürich, Switzerland.; Department of Visceral and Transplantation Surgery, University of Bern, 3012 Bern, Switzerland.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Apr 25; Vol. 19 (3). Date of Electronic Publication: 2024 Apr 25.
DOI:	10.1088/1748-605X/ad3da4
Abstrakt:	The standard surgical procedure for abdominal hernia repair with conventional prosthetic mesh still results in a high recurrence rate. In the present study, we propose a fibroblast matrix implant (FMI), which is a three-dimensional (3D) poly-L-lactic acid scaffold coated with collagen (matrix) and seeded with fibroblasts, as an alternative mesh for hernia repair. The matrix was seeded with fibroblasts (cellularized) and treated with a conditioned medium (CM) of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC). Fibroblast proliferation and function were assessed and compared between treated with CM hUC-MSC and untreated group, 24 h after seeding onto the matrix ( n = 3). To study the matrices in vivo, the hernia was surgically created on male Sprague Dawley rats and repaired with four different grafts ( n = 3), including a commercial mesh (mesh group), a matrix without cells (cell-free group), a matrix seeded with fibroblasts (FMI group), and a matrix seeded with fibroblasts and cultured in medium treated with 1% CM hUC-MSC (FMI-CM group). In vitro examination showed that the fibroblasts' proliferation on the matrices (treated group) did not differ significantly compared to the untreated group. CM hUC-MSC was able to promote the collagen synthesis of the fibroblasts, resulting in a higher collagen concentration compared to the untreated group. Furthermore, the in vivo study showed that the matrices allowed fibroblast growth and supported cell functionality for at least 1 month after implantation. The highest number of fibroblasts was observed in the FMI group at the 14 d endpoint, but at the 28 d endpoint, the FMI-CM group had the highest. Collagen deposition area and neovascularization at the implantation site were observed in all groups without any significant difference between the groups. FMI combined with CM hUC-MSC may serve as a better option for hernia repair, providing additional reinforcement which in turn should reduce hernia recurrence. (© 2024 IOP Publishing Ltd.)
Databáze:	MEDLINE
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