Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California.

Autor: Maguvu TE; Department of Plant Pathology, University of California, Davis, Davis, CA, United States of America.; Kearney Agricultural Research and Extension Center, Parlier, CA, United States of America., Frias RJ; Department of Plant Pathology, University of California, Davis, Davis, CA, United States of America., Hernandez-Rosas AI; Department of Plant Pathology, University of California, Davis, Davis, CA, United States of America., Holtz BA; University of California Cooperative Extension, CA, United States of America., Niederholzer FJA; University of California Cooperative Extension, CA, United States of America., Duncan RA; University of California Cooperative Extension, CA, United States of America., Yaghmour MA; University of California Cooperative Extension, CA, United States of America., Culumber CM; University of California Cooperative Extension, CA, United States of America., Gordon PE; University of California Cooperative Extension, CA, United States of America., Vieira FCF; Department of Plant Pathology and Microbiology, University of California, Riverside, Riverside, CA, United States of America., Rolshausen PE; Department of Plant Pathology and Microbiology, University of California, Riverside, Riverside, CA, United States of America., Adaskaveg JE; Department of Plant Pathology and Microbiology, University of California, Riverside, Riverside, CA, United States of America., Burbank LP; U.S. Department of Agriculture, Agricultural Research Service, Parlier, CA, United States of America., Lindow SE; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United States of America., Trouillas FP; Department of Plant Pathology, University of California, Davis, Davis, CA, United States of America.; Kearney Agricultural Research and Extension Center, Parlier, CA, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 11; Vol. 19 (4), pp. e0297867. Date of Electronic Publication: 2024 Apr 11 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0297867
Abstrakt: We sequenced and comprehensively analysed the genomic architecture of 98 fluorescent pseudomonads isolated from different symptomatic and asymptomatic tissues of almond and a few other Prunus spp. Phylogenomic analyses, genome mining, field pathogenicity tests, and in vitro ice nucleation and antibiotic sensitivity tests were integrated to improve knowledge of the biology and management of bacterial blast and bacterial canker of almond. We identified Pseudomonas syringae pv. syringae, P. cerasi, and P. viridiflava as almond canker pathogens. P. syringae pv. syringae caused both canker and foliar (blast) symptoms. In contrast, P. cerasi and P. viridiflava only caused cankers, and P. viridiflava appeared to be a weak pathogen of almond. Isolates belonging to P. syringae pv. syringae were the most frequently isolated among the pathogenic species/pathovars, composing 75% of all pathogenic isolates. P. cerasi and P. viridiflava isolates composed 8.3 and 16.7% of the pathogenic isolates, respectively. Laboratory leaf infiltration bioassays produced results distinct from experiments in the field with both P. cerasi and P. syringae pv. syringae, causing significant necrosis and browning of detached leaves, whereas P. viridiflava conferred moderate effects. Genome mining revealed the absence of key epiphytic fitness-related genes in P. cerasi and P. viridiflava genomic sequences, which could explain the contrasting field and laboratory bioassay results. P. syringae pv. syringae and P. cerasi isolates harboured the ice nucleation protein, which correlated with the ice nucleation phenotype. Results of sensitivity tests to copper and kasugamycin showed a strong linkage to putative resistance genes. Isolates harbouring the ctpV gene showed resistance to copper up to 600 μg/ml. In contrast, isolates without the ctpV gene could not grow on nutrient agar amended with 200 μg/ml copper, suggesting ctpV can be used to phenotype copper resistance. All isolates were sensitive to kasugamycin at the label-recommended rate of 100μg/ml.
Competing Interests: The authors have declared that no competing interests exist
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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