Protocol to detect senescence-associated β-galactosidase and immunoperoxidase activity in fresh-frozen murine tissues.

Autor:	Idelfonso-García OG; Laboratory of Liver Diseases, National Institute of Genomic Medicine - INMEGEN, Mexico City 14610, Mexico; Department of Health Sciences, Metropolitan Autonomous University - Iztapalapa Campus - UAM-I, Mexico City 09340, Mexico. Electronic address: oidelfonso@inmegen.edu.mx., Pacheco-Rivera R; Laboratory of Molecular Diagnostics, Department of Biochemistry, National School of Biological Sciences of the National Polytechnic Institute, Mexico City 07738, Mexico., Alarcón-Sánchez BR; Laboratory of Liver Diseases, National Institute of Genomic Medicine - INMEGEN, Mexico City 14610, Mexico; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute - CINVESTAV-IPN, Mexico City 07360, Mexico., Serrano-Luna J; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute - CINVESTAV-IPN, Mexico City 07360, Mexico., Baltiérrez-Hoyos R; Laboratory of Fibrosis and Cancer, Faculty of Medicine and Surgery, 'Benito Juárez' Autonomous University of Oaxaca - UABJO, Mexico City 68120, Mexico; Deputy Directorate of Humanistic and Scientific Research, National Council of Humanities, Sciences and Technologies - CONAHCYT, Mexico City 03940, Mexico., Vásquez-Garzón VR; Laboratory of Fibrosis and Cancer, Faculty of Medicine and Surgery, 'Benito Juárez' Autonomous University of Oaxaca - UABJO, Mexico City 68120, Mexico; Deputy Directorate of Humanistic and Scientific Research, National Council of Humanities, Sciences and Technologies - CONAHCYT, Mexico City 03940, Mexico., Muriel P; Laboratory of Experimental Hepatology, Department of Pharmacology, Center for Research and Advanced Studies of the National Polytechnic Institute - CINVESTAV-IPN, Mexico City 07360, Mexico., Villa-Treviño S; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute - CINVESTAV-IPN, Mexico City 07360, Mexico., Pérez-Carreón JI; Laboratory of Liver Diseases, National Institute of Genomic Medicine - INMEGEN, Mexico City 14610, Mexico., Arellanes-Robledo J; Laboratory of Liver Diseases, National Institute of Genomic Medicine - INMEGEN, Mexico City 14610, Mexico; Deputy Directorate of Humanistic and Scientific Research, National Council of Humanities, Sciences and Technologies - CONAHCYT, Mexico City 03940, Mexico. Electronic address: jarellanes@inmegen.gob.mx.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103009. Date of Electronic Publication: 2024 Apr 10.
DOI:	10.1016/j.xpro.2024.103009
Abstrakt:	Double labeling to identify different markers in the same tissue section represents a useful tool either for in situ diagnosis or characterization of molecular associations. Here, we present a protocol to detect senescence-associated β-galactosidase (SA-βGal) and immunoperoxidase (IPO) activity in fresh-frozen murine tissues. We describe steps for tissue collection, solution preparation, SA-βGal staining, IPO staining, hematoxylin counterstaining, microscopic observation, and signal quantification. This protocol can be used to detect in situ proteins alongside SA-βGal activity. For complete details on the use and execution of this protocol, please refer to Pacheco-Rivera et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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