Analysis of the Cadmium Removal Mechanism of Human Gut Bacteria Enterococcus faecalis Strain ATCC19433 from a Genomic Perspective.

Autor: Zheng Y; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.; Guizhou Ecological Environment Resources Management Co., LTD, Guiyang, 550009, China., Duan Z; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.; School of Public Health, Zunyi Medical University, Zunyi, 563006, China., Wu Y; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China. ygwu72@126.com.; Guizhou Hostile Environment Ecological Restoration Technology Engineering Research Centre, Guiyang, 550025, China. ygwu72@126.com.; Guizhou Karst Environmental Ecosystem Observation and Research Station, Ministry of Education, Guiyang, 550025, China. ygwu72@126.com., Luo Y; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China., Peng X; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China., Wu J; College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2024 Apr 11. Date of Electronic Publication: 2024 Apr 11.
DOI: 10.1007/s12011-024-04169-6
Abstrakt: Cadmium (Cd) is one of the most well-known toxic metals capable of entering the human body via the food chain, leading to serious health problems. Human gut microbes play a pivotal role in controlling Cd bioavailability and toxicity within the human gastrointestinal tract, primarily due to their capacity for Cd adsorption and metabolism. In this work, a Cd-resistant bacterial strain, Enterococcus faecalis strain ATCC19433 was isolated from human gut microbiota. Cd binding assays and comprehensive characterization analyses were performed, revealing the ability of strain ATCC19433 to remove Cd from the solution. Cd adsorption primarily occurred on the bacterial cell walls, which was ascribed to the exciting of functional groups on the bacterial surfaces, containing alkyl, amide II, and phosphate groups; meanwhile, Cd could enter cells, probably through transport channels or via diffusion. These results indicated that Cd removal by the strain was predominantly dependent on biosorption and bioaccumulation. Whole-genome sequencing analyses further suggested the probable mechanisms of biosorption and bioaccumulation, including Cd transport by transporter proteins, active efflux of Cd by cadmium efflux pumps, and mitigating oxidative stress-induced cell damage by DNA repair proteases. This study evaluated the Cd removal capability and mechanism of Enterococcus faecalis strain ATCC19433 while annotating the genetic functions related to Cd removal, which may facilitate the development of potential human gut strains for the removal of Cd.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: