Field experience with the 8-HPV-type oncoprotein test for cervical cancer screening among HPV-positive women living with and without HIV in LMICs.

Autor: Downham L; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Rol ML; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Forestier M; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Romero P; Instituto Nacional de Cancerología, Bogota, Colombia., Mendoza L; Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay., Mongelós P; Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay., Picconi MA; Instituto Nacional de Enfermedades Infecciosas-ANLIS Dr Malbrán, Buenos Aires, Argentina., Colucci MC; Instituto Nacional de Enfermedades Infecciosas-ANLIS Dr Malbrán, Buenos Aires, Argentina., Padin VM; Instituto Nacional de Enfermedades Infecciosas-ANLIS Dr Malbrán, Buenos Aires, Argentina., Flores AP; Agencia Costarricense de Investigaciones Biomédicas (ACIB), Fundación Inciensa, Guanacaste, Costa Rica., Zúñiga M; Agencia Costarricense de Investigaciones Biomédicas (ACIB), Fundación Inciensa, Guanacaste, Costa Rica., Ferrera A; Instituto de Investigaciones en Microbiología, Escuela de Microbiología, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., Cabrera Y; Instituto de Investigaciones en Microbiología, Escuela de Microbiología, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., Crispín MF; Facultad de Medicina, Universidad Mayor, Real y Pontificia de San Francisco Xavier de Chuquisaca, Sucre, Bolivia., Ramirez AT; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Cele L; University of Kwazulu Natal, Durban, South Africa., Diop-Ndiaye H; Laboratoire Bactériologie-Virologie, CHU Aristide le Dantec, Dakar, Senegal., Samaté D; Laboratoire Bactériologie-Virologie, CHU Aristide le Dantec, Dakar, Senegal., Manga P; Laboratoire Bactériologie-Virologie, CHU Aristide le Dantec, Dakar, Senegal., Thiam FB; Laboratoire Bactériologie-Virologie, CHU Aristide le Dantec, Dakar, Senegal., Rodriguez MI; Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo, Paraguay., DSouza JP; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Nyaga VN; Department of Cancer Epidemiology, Scientific Institute of Public Health, Brussels, Belgium., Diop M; Laboratoire Bactériologie-Virologie, CHU Aristide le Dantec, Dakar, Senegal., Sebitloane M; University of Kwazulu Natal, Durban, South Africa., Sánchez GI; Grupo Infección y Cáncer, Universidad de Antioquia, Medellin, Colombia., Teran C; Facultad de Medicina, Universidad Mayor, Real y Pontificia de San Francisco Xavier de Chuquisaca, Sucre, Bolivia., Calderon A; Caja Costarricense del Seguro Social, Puntarenas, Costa Rica., Wiesner C; Instituto Nacional de Cancerología, Bogota, Colombia., Murillo R; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France.; Centro Javeriano de Oncología, Hospital Universitario San Ignacio, Bogotá, Colombia., Herrero R; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France.; Caja Costarricense del Seguro Social, Puntarenas, Costa Rica., Baena A; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France., Almonte M; Early Detection, Prevention and Infections Branch, International Agency for Research on Cancer, Lyon, France.
Jazyk: angličtina
Zdroj: International journal of cancer [Int J Cancer] 2024 Sep 01; Vol. 155 (5), pp. 816-827. Date of Electronic Publication: 2024 Apr 11.
DOI: 10.1002/ijc.34953
Abstrakt: Overexpression of HPV-oncoproteins E6 and E7 is necessary for HPV-driven cervical carcinogenesis. Hence, these oncoproteins are promising disease-specific biomarkers. We assessed the technical and operational characteristics of the 8-HPV-type OncoE6/E7 Cervical Test in different laboratories using cervical samples from HPV-positive women living with (WLWH) and without HIV. The 8-HPV-type OncoE6/E7 Test (for short: "OncoE6/E7 test") was performed in 2833 HIV-negative women and 241 WLWH attending multicentric studies in Latin America (ESTAMPA study), and in Africa (CESTA study). Oncoprotein positivity were evaluated at each testing site, according to HIV status as well as type-specific agreement with HPV-DNA results. A feedback questionnaire was given to the operators performing the oncoprotein test to evaluate their impression and acceptability regarding the test. The OncoE6/E7 test revealed a high positivity rate heterogeneity across all testing sites (I 2 : 95.8%, p < .01) with significant lower positivity in WLWH compared to HIV-negative women (12% vs 25%, p < .01). A similar HPV-type distribution was found between HPV DNA genotyping and oncoprotein testing except for HPV31 and 33 (moderate agreement, k = 0.57). Twenty-one laboratory technicians were trained on oncoprotein testing. Despite operators' concerns about the time-consuming procedure and perceived need for moderate laboratory experience, they reported the OncoE6/E7 test as easy to perform and user-friendly for deployment in resource-limited settings. The high positivity rate variability found across studies and subjectivity in test outcome interpretation could potentially results in oncoprotein false positive/negative, and thus the need for further refinements before implementation of the oncoprotein testing in screen-triage-and-treat approaches is warranted.
(© 2024 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC.)
Databáze: MEDLINE
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