Osteogenic differentiation of human mesenchymal stem cells on electroactive substrates.

Autor:	Tamaño-Machiavello MN; Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 València, Spain., Carvalho EO; Centre of Physics, Universidade do Minho, 4710-057, Braga, Portugal., Correia D; Centre of Chemistry, University of Minho, 4710-057, Braga, Portugal., Cordón L; Hematology Research Group, Instituto de Investigación Sanitaria La Fe, València, Spain.; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, Madrid, Spain., Lanceros-Méndez S; Centre of Physics, Universidade do Minho, 4710-057, Braga, Portugal.; BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940, Leioa, Spain.; IKERBASQUE, Basque Foundation for Science, 48009, Bilbao, Spain., Sempere A; Hematology Research Group, Instituto de Investigación Sanitaria La Fe, València, Spain.; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, Madrid, Spain.; Hematology Department, Hospital Universitario y Politécnico La Fe, València, Spain., Sabater I Serra R; Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 València, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain., Ribelles JLG; Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 València, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Mar 29; Vol. 10 (7), pp. e28880. Date of Electronic Publication: 2024 Mar 29 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e28880
Abstrakt:	This study investigates the effect of electroactivity and electrical charge distribution on the biological response of human bone marrow stem cells (hBMSCs) cultured in monolayer on flat poly(vinylidene fluoride), PVDF, substrates. Differences in cell behaviour, including proliferation, expression of multipotency markers CD90, CD105 and CD73, and expression of genes characteristic of different mesenchymal lineages, were observed both during expansion in basal medium before reaching confluence and in confluent cultures in osteogenic induction medium. The crystallisation of PVDF in the electrically neutral α-phase or in the electroactive phase β, both unpoled and poled, has been found to have an important influence on the biological response. In addition, the presence of a permanent positive or negative surface electrical charge distribution in phase β substrates has also shown a significant effect on cell behaviour. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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