Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study.

Autor:	Pascual S; bioMérieux, Marcy l'Etoile, France., Noble B; bioMérieux, Salt Lake City, USA., Ahmad-Saeed N; University Hospital Southampton NHS Foundation Trust, Southampton, United Kingdom., Aldridge C; Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, United Kingdom., Ambretti S; S. Orsola Bologna, Microbiology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy., Amit S; Sheba Medical Center, Ramat Gan, Israel., Annett R; University Hospital of Wales, Cardiff, Wales, United Kingdom., O'Shea SA; University Hospital Southampton NHS Foundation Trust, Southampton, United Kingdom., Barbui AM; San Giovanni Battista, Department of Public Health and Pediatrics Microbiology and Virology Unit, Città della Salute e della Scienza, Turin, Italy., Barlow G; Hull University Teaching Hospitals NHS Trust, Hull, United Kingdom., Barrett L; Oxford University Hospitals (OUH), Oxford, United Kingdom., Berth M; AZ Alma, Eeklo, Belgium., Bondi A; Department of Public Health and Pediatrics, University of Turin, Turin, Italy., Boran N; Mater Misericordiae University Hospital, Dublin, Ireland., Boyd SE; Chelsea and Westminster NHS Foundation Trust, London, United Kingdom., Chaves C; Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal., Clauss M; University Hospital Basel, Basel, Switzerland., Davies P; NHS Greater Glasgow and Clyde, Glasgow Royal Infirmary, University of Glasgow, Glasgow, United Kingdom., Dianzo-Delgado IT; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., Esteban J; Dept. of Clinical Microbiology, IIS-Fundación Jiménez Díaz, CIBERINFEC-CIBER de Enfermedades Infecciosas, Madrid, Spain., Fuchs S; Institute of Hygiene and Medical Microbiology Medizinische Universität Innsbruck, Innsbruck, Austria., Friis-Hansen L; Copenhagen University Hospital, Bispebjerg, Copenhagen, Denmark.; Dept. Clinical Microbiology at Rigshospitalet, Copenhagen, Denmark., Goldenberger D; University Hospital Basel, Basel, Switzerland., Golle A; NZOLH Maribor, Maribor, Slovenia., Groonroos JO; Varsinais-Suomen sairaanhoitopiiri, Loimaa, Finland., Hoffmann I; MVZ Labor Dr. Reising-Ackermann und Kollegen, Limbach Leipzig, Germany., Hoffmann T; Sheba Medical Center, Ramat Gan, Israel., Hughes H; University Hospital of Wales, Cardiff, Wales, United Kingdom., Ivanova M; East Tallinn Central Hospital, Tallin, Estonia., Jezek P; Regional Hospital Příbram, Příbram, Czech Republic., Jones G; University Hospital of Wales, Cardiff, Wales, United Kingdom., Ceren Karahan Z; Ankara University School of Medicine Department of Medical Microbiology, Ankara, Türkiye., Lass-Flörl C; Institute of Hygiene and Medical Microbiology Medizinische Universität Innsbruck, Innsbruck, Austria., Laurent F; Hospices Civils de Lyon, Lyon, France., Leach L; Oxford University Hospitals (OUH), Oxford, United Kingdom., Horsbøll Pedersen ML; Copenhagen University Hospital, Bispebjerg, Copenhagen, Denmark.; Dept. Clinical Microbiology at Rigshospitalet, Copenhagen, Denmark., Loiez C; Centre Hospitalier Universitaire de Lille, Lille, France., Lynch M; Mater Misericordiae University Hospital, Dublin, Ireland., Maloney RJ; Hull University Teaching Hospitals NHS Trust, Hull, United Kingdom., Marsh M; Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, United Kingdom., Milburn O; Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, United Kingdom., Mitchell S; University Hospital of Wales, Cardiff, Wales, United Kingdom., Moore LSP; Chelsea and Westminster NHS Foundation Trust, London, United Kingdom., Moffat L; NHS Greater Glasgow and Clyde, Glasgow Royal Infirmary, University of Glasgow, Glasgow, United Kingdom., Murdjeva M; University Hospital 'St George', Plovdiv, Bulgaria., Murphy ME; NHS Greater Glasgow and Clyde, Glasgow Royal Infirmary, University of Glasgow, Glasgow, United Kingdom., Nayar D; Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, United Kingdom., Nigrisoli G; S. Orsola Bologna, Microbiology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy., O'Sullivan F; Mater Misericordiae University Hospital, Dublin, Ireland., Öz B; Ankara University School of Medicine Department of Medical Microbiology, Ankara, Türkiye., Peach T; University Hospital of Wales, Cardiff, Wales, United Kingdom., Petridou C; Hampshire Hospitals NHS Foundation Trust, Winchester, UK., Prinz M; Krankenhaus Göttlicher Heiland, Vienna, Austria., Rak M; Koper lab, Orthopedic Hospital Valdoltra, Valdoltra, Slovenia., Reidy N; Mater Misericordiae University Hospital, Dublin, Ireland., Rossolini GM; Careggi University Hospital, Florence, Italy., Roux AL; Hôpital Ambroise Paré, APHP, Boulogne-Billancourt, France., Ruiz-Garbajosa P; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III. Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain., Saeed K; University Hospital Southampton NHS Foundation Trust, Southampton, United Kingdom., Salar-Vidal L; Dept. of Clinical Microbiology, IIS-Fundación Jiménez Díaz, CIBERINFEC-CIBER de Enfermedades Infecciosas, Madrid, Spain., Salas Venero C; Hospital Universitario Marqués de Valdecilla, Santander, Spain., Selvaratnam M; Oxford University Hospitals (OUH), Oxford, United Kingdom., Senneville E; Centre Hospitalier Universitaire de Lille, Lille, France., Starzengruber P; Allgemeines Krankenhaus Wien, Vienna, Austria., Talbot B; NHS Greater Glasgow and Clyde, Glasgow Royal Infirmary, University of Glasgow, Glasgow, United Kingdom., Taylor V; University Hospital of Wales, Cardiff, Wales, United Kingdom., Trebše R; Koper lab, Orthopedic Hospital Valdoltra, Valdoltra, Slovenia., Wearmouth D; Hull University Teaching Hospitals NHS Trust, Hull, United Kingdom., Willinger B; Allgemeines Krankenhaus Wien, Vienna, Austria., Wouthuyzen-Bakker M; University Medical Center Groningen, Groningen, the Netherlands., Couturier B; bioMérieux, Salt Lake City, USA., Allantaz F; bioMérieux, Marcy l'Etoile, France.
Jazyk:	angličtina
Zdroj:	Journal of bone and joint infection [J Bone Jt Infect] 2024 Feb 28; Vol. 9 (1), pp. 87-97. Date of Electronic Publication: 2024 Feb 28 (Print Publication: 2024).
DOI:	10.5194/jbji-9-87-2024
Abstrakt:	Introduction : The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods : A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results : A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus , Streptococcus species, Enterococcus faecalis , Kingella kingae , Neisseria gonorrhoeae , and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion : The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting. Competing Interests: At least one of the (co-)authors is a member of the editorial board of Journal of Bone and Joint Infection. The peer-review process was guided by an independent editor, and the authors also have no other competing interests to declare. (Copyright: © 2024 Stéphanie Pascual et al.)
Databáze:	MEDLINE
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