Role of Rmd9p in 3'-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae.

Autor: Singh J; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India., Singh S; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India., Emam EAF; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India., Varshney U; Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India. Electronic address: varshney@iisc.ac.in.
Jazyk: angličtina
Zdroj: Mitochondrion [Mitochondrion] 2024 May; Vol. 76, pp. 101876. Date of Electronic Publication: 2024 Apr 08.
DOI: 10.1016/j.mito.2024.101876
Abstrakt: Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5'-end processing uses Ccm1p and Pet127p, the mechanisms of the 3'-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3'-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3'-end, and in the loss of the 3'-minor domain. Rmd9p binds to the sequences in the 3'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3'-end spacer processing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
Databáze: MEDLINE
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