The beneficial effects of quinoa seed extract supplementation on ram sperm quality following cryopreservation.

Autor: Khalil WA; Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt. Electronic address: w-khalil@mans.edu.eg., Hassan MAE; Animal Production Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza 12619, Egypt., Ibrahim S; Department of Animal Reproduction and AI, Veterinary Research Institute, National Research Centre, Dokki, 12622, Egypt., Mohammed AK; Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt., El-Harairy MA; Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt., Abdelnour SA; Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt. Electronic address: samehtimor86@gmail.com.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2024 May; Vol. 264, pp. 107472. Date of Electronic Publication: 2024 Apr 04.
DOI: 10.1016/j.anireprosci.2024.107472
Abstrakt: Although cryopreservation is a reliable method used in assisted reproduction to preserve genetic materials, it can stimulate the occurrence of oxidative stress, which affects sperm structure and function. This research was conducted to explore the effects of quinoa seed extracts (QSE) on ram sperm quality, oxidative biomarkers, and the gene expression of frozen-thawed ram sperm. Semen samples were diluted in extenders supplemented with 0 (QSE0), 250 (QSE1), 500 (QSE2), 750 (QSE3), and 1000 (QSE4) µg of QSE /mL, and then frozen according to the typical procedure. The findings indicate that the QSE3 and QSE4 groups provided the optimal results in terms of sperm viability and progressive motility. Sperm kinematics were considerably enhanced in the QSE3 group compared to the other groups (P<0.01). QSE (500-1000 µg/mL) significantly decreased the apoptosis-like changes (higher viable and lower apoptotic sperm) in ram sperm (P<0.001). The percentage of live sperm with intact acrosomes was significantly increased, while the percentage of detached and intact acrosomes in live and dead sperm were significantly decreased respectively by the QSE addition (P<0.001). All QSE groups had higher TAC and lower MDA and H 2 O 2 levels than the control group (P<0.001). The expressions of SOD1, CAT, GABPB1, and GPX1 genes in sperm samples were significantly increased, while the CASP3 gene was significantly decreased in all QSE-supplemented samples. Our data suggest that QSE has beneficial effects on sperm quality of cryopreserved ram semen, which are achieved by promoting sperm antioxidant-related genes and reducing apoptosis-related gene.
Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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