The N-terminal domains of NLR immune receptors exhibit structural and functional similarities across divergent plant lineages.

Autor:	Chia KS; Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK., Kourelis J; The Sainsbury Laboratory, University of East Anglia, Norwich NR4 7UH, UK., Teulet A; Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK., Vickers M; Computational and Systems Biology, John Innes Centre, Norwich NR4 7UH, UK., Sakai T; The Sainsbury Laboratory, University of East Anglia, Norwich NR4 7UH, UK., Walker JF; Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA., Schornack S; Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK., Kamoun S; The Sainsbury Laboratory, University of East Anglia, Norwich NR4 7UH, UK., Carella P; Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Jul 02; Vol. 36 (7), pp. 2491-2511.
DOI:	10.1093/plcell/koae113
Abstrakt:	Nucleotide-binding domain and leucine-rich repeat (NLR) proteins are a prominent class of intracellular immune receptors in plants. However, our understanding of plant NLR structure and function is limited to the evolutionarily young flowering plant clade. Here, we describe an extended spectrum of NLR diversity across divergent plant lineages and demonstrate the structural and functional similarities of N-terminal domains that trigger immune responses. We show that the broadly distributed coiled-coil (CC) and toll/interleukin-1 receptor (TIR) domain families of nonflowering plants retain immune-related functions through translineage activation of cell death in the angiosperm Nicotiana benthamiana. We further examined a CC subfamily specific to nonflowering lineages and uncovered an essential N-terminal MAEPL motif that is functionally comparable with motifs in resistosome-forming CC-NLRs. Consistent with a conserved role in immunity, the ectopic activation of CCMAEPL in the nonflowering liverwort Marchantia polymorpha led to profound growth inhibition, defense gene activation, and signatures of cell death. Moreover, comparative transcriptomic analyses of CCMAEPL activity delineated a common CC-mediated immune program shared across evolutionarily divergent nonflowering and flowering plants. Collectively, our findings highlight the ancestral nature of NLR-mediated immunity during plant evolution that dates its origin to at least ∼500 million years ago. Competing Interests: Conflict of interest statement S.K. receives funding from industry on NLR biology. S.K. has filed patents on NLR biology. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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