BRAF and RET polymorphism association with thyroid cancer risk, a preliminary study from Khyber Pakhtunkhwa population.

Autor: Batool M; Institute of Biotechnology & Genetic Engineering (Health Division), The University of Agriculture Peshawar, P.O. Box: 25130, Peshawar, Pakistan., Khan NU; Institute of Biotechnology & Genetic Engineering (Health Division), The University of Agriculture Peshawar, P.O. Box: 25130, Peshawar, Pakistan. najeebkhan@aup.edu.pk., Khan H; Institute of Biotechnology & Genetic Engineering (Health Division), The University of Agriculture Peshawar, P.O. Box: 25130, Peshawar, Pakistan., Almutairi MH; Zoology Department, College of Science, King Saud University, P.O. Box: 2455, Riyadh, 11451, Saudi Arabia., Ali I; Centre for Applied Mathematics and Bioinformatics (CAMB), Gulf University for Science and Technology, Hawally, Kuwait., Adams BD; Department of RNA Sciences, The Brain Institute of America, New Haven, CT, USA.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Apr 10; Vol. 51 (1), pp. 502. Date of Electronic Publication: 2024 Apr 10.
DOI: 10.1007/s11033-024-09480-y
Abstrakt: Background: Thyroid cancer, originating in the neck's thyroid gland, encompasses various types. Genetic mutations, particularly in BRAF and RET genes are crucial in its development. This study investigates the association between BRAF (rs113488022) and RET (rs77709286) polymorphisms and thyroid cancer risk in the Khyber Pakhtunkhwa (KP) population.
Methods: Blood samples from 100 thyroid cancer patients and 100 healthy controls were genotyped using ARMS-PCR followed by gel electrophoresis and statistical analysis.
Results: Analysis revealed a significant association between the minor allele T of BRAF (rs113488022) and thyroid cancer risk (P = 0.0001). Both genotypes of BRAF (rs113488022) showed significant associations with thyroid cancer risk (AT; P = 0.0012 and TT; P = 0.045). Conversely, the minor allele G of RET (rs77709286) exhibited a non-significant association with thyroid cancer risk (P = 0.2614), and neither genotype showed significant associations (CG; P = 0.317, GG; P = 0.651). Demographic and clinical parameters analysis using SPSS showed a non-significant association between BRAF and RET variants and age group (P = 0.878 and P = 0.536), gender (P = 0.587 and P = 0.21), tumor size (P = 0.796 and P = 0.765), or tumor localization (P = 0.689 and P = 0.727).
Conclusion: In conclusion, this study emphasizes the significant association between BRAF polymorphism and thyroid cancer risk, while RET polymorphism showed a less pronounced impact. Further validation using larger and specific datasets is essential to establish conclusive results.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
Externí odkaz: