Use of cytobrush for bacteriological and cytological diagnosis of endometritis in mares.

Autor:	Prete CD; Reproduction Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Nocera FP; Diagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Piegari G; Diagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Palumbo V; Regional Reference Center for Urban Veterinary Hygiene (CRIUV), Naples, Italy., Martino L; Diagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Cocchia N; Reproduction Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Paciello O; Diagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Montano C; Surgery Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy., Pasolini MP; Surgery Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Jazyk:	angličtina
Zdroj:	Veterinary world [Vet World] 2024 Feb; Vol. 17 (2), pp. 398-406. Date of Electronic Publication: 2024 Feb 16.
DOI:	10.14202/vetworld.2024.398-406
Abstrakt:	Background and Aim: A combined microbial and cytological examination of uterine samples is the main diagnostic method for endometritis in mares. This study aimed to describe a procedure for using the same uterine cytobrush (CB) for both bacteriological and cytological evaluation. Material and Methods: The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet. Results: The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results. Conclusion: The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts. Competing Interests: The authors declare that they have no competing interests. (Copyright: © Prete, et al.)
Databáze:	MEDLINE
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