Detection of Campylobacter spp. in farmed sheep in Mymensingh division of Bangladesh: Prevalence, risk factors, and antimicrobial susceptibility status.

Autor: Nobi MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Haque AKMZ; Kazi Farms Poultry Laboratory, Holding no-8/1, Floor no-A3 and A4, Padma Plaza (Opposite of Gazipur Commerce College), Chandana - Chowrasta, Gazipur-1704, Bangladesh., Islam MR; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Islam SS; Department of Livestock Services, Ministry of Fisheries and Livestock, Dhaka-1215, Bangladesh., Arif M; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Sikder MH; Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh., Kabir SML; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2024 Feb; Vol. 17 (2), pp. 245-254. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.14202/vetworld.2024.245-254
Abstrakt: Background and Aim: Campylobacter infections in sheep may be asymptomatic or cause enteritis, ileitis, infertility, and abortion. Thus, this study aimed to estimate the prevalence of Campylobacter spp. in farming sheep and to detect risk factors, molecular patterns, and antimicrobial susceptibility status of these pathogens.
Materials and Methods: Four hundred and eight fecal samples were collected from 12 flocks in the Mymensingh and Sherpur districts. Samples were tested by both basic (culture and biochemical tests) and molecular (initially 16S rRNA and later hipO gene-based polymerase chain reaction). Furthermore, the antimicrobial susceptibility status of Campylobacter jejuni was confirmed using disk diffusion. Flock- and animal-level data were captured using semi-structured interviews with farm owners under bivariate and multivariate logistic regression analyses to confirm the risk factors for Campylobacter -positive status.
Results: The prevalence of C. jejuni staining at the animal and flock levels was 8.82% (36/408) and 66.70% (8/12), respectively. The age of sheep was identified as an important risk factor. Up to 1 year of age, sheep were 3.78 times more likely to be infected with C. jejuni (95% confidence interval: 1.0736-13.3146, p = 0.038). Of the 36 isolates of C. jejuni , all were found to be fully susceptible (100%) to gentamicin and ciprofloxacin. In this study, three antimicrobial agents, oxytetracycline, azithromycin, and ceftriaxone, were fully resistant (100%). The majority of isolates were resistant to a combination of 4-6 antimicrobial agents.
Conclusion: The present study highlights the predominant maintenance of zoonotic Campylobacter species in sheep, and their burden on human health is enormous. Therefore, environmental, animal, and human health needs to be focused under a One Health lens to mitigate the occurrence of Campylobacter in farm settings and to prevent further introduction to animals and humans.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © Nobi, et al.)
Databáze: MEDLINE
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