First Molecular Detection of SARS-CoV-2 in Sewage and Wastewater in Ghana.

Autor: Duker EO; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Obodai E; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Addo SO; Parasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Kwasah L; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Mensah ES; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Gberbi E; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Anane A; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Attiku KO; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Boakye J; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Agbotse GD; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Dickson AE; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Quarcoo JA; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Darko PA; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Larbi YA; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Ntim NAA; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana., Dzudzor B; Department of Medical Biochemistry, University of Ghana Medical School, University of Ghana, Legon, Accra, Ghana., Odoom JK; Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2024 Mar 25; Vol. 2024, pp. 9975781. Date of Electronic Publication: 2024 Mar 25 (Print Publication: 2024).
DOI: 10.1155/2024/9975781
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is shed in the stool of infected individuals and can be detected in sewage and wastewater contaminated with infected stool. This study is aimed at detecting the virus and its potential survival in sewage and wastewater in Ghana. The cross-sectional study included samples from 16 validated environmental surveillance sites in 7 regions of Ghana. A total of 354 samples composed of wastewater (280) and sewage (74) were collected from November 2020 to November 2022. Overall, 17% of the samples were positive for SARS-CoV-2 by real-time PCR, with 6% in sewage and 11% in wastewater. The highest number of positive samples was collected from the Greater Accra Region (7.3%) with the least recorded in the Bono East Region (0.6%). Further characterization of the positive samples using the next-generation sequencing (NGS) approach yielded two variants: Alpha (B.1.1.7) and Delta (AY.36). Attempts to isolate SARS-CoV-2 in the Vero cell line were not successful probably due to the low viral load concentrations (Ct values > 35) or prolonged exposure to high temperatures rendering the virus noninfectious. Our findings suggest that SARS-CoV-2 RNA in sewage and wastewater may not be infectious, but the prevalence shows that the virus persists in the communities within Ghana.
Competing Interests: The authors declare no conflict of interest.
(Copyright © 2024 Ewurabena Oduma Duker et al.)
Databáze: MEDLINE
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