Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis.

Autor: Chen JP; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, People's Republic of China.; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China., Zhou L; Jiangsu Nannong High-Tech Co., Ltd., Jiangyin, People's Republic of China., Gong JS; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, People's Republic of China.; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China., Wang NK; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China., Miao FF; Jiangsu Nannong High-Tech Co., Ltd., Jiangyin, People's Republic of China., Su C; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, People's Republic of China.; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China., Gao XL; Jiangsu Nannong High-Tech Co., Ltd., Jiangyin, People's Republic of China., Xu GQ; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China., Shi JS; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, People's Republic of China.; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China., Xu ZH; Institute of Future Food Technology, JITRI, Yixing, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.; College of Biomass Science and Engineering, Sichuan University, Chengdu, People's Republic of China.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2024 Jul; Vol. 121 (7), pp. 2163-2174. Date of Electronic Publication: 2024 Apr 10.
DOI: 10.1002/bit.28710
Abstrakt: Pathogenic bacterial membrane proteins (MPs) are a class of vaccine and antibiotic development targets with widespread clinical application. However, the inherent hydrophobicity of MPs poses a challenge to fold correctly in living cells. Herein, we present a comprehensive method to improve the soluble form of MP antigen by rationally designing multi-epitope chimeric antigen (ChA) and screening two classes of protein-assisting folding element. The study uses a homologous protein antigen as a functional scaffold to generate a ChA possessing four epitopes from transferrin-binding protein A of Glaesserella parasuis. Our engineered strain, which co-expresses P17 tagged-ChA and endogenous chaperones groEL-ES, yields a 0.346 g/L highly soluble ChA with the property of HPS-positive serum reaction. Moreover, the protein titer of ChA reaches 4.27 g/L with >90% soluble proportion in 5-L bioreactor, which is the highest titer reported so far. The results highlight a timely approach to design and improve the soluble expression of MP antigen in industrially viable applications.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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