| Autor: |
Colley ME; 1Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA; email: jeff.spraggins@vanderbilt.edu.; 2Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA., Esselman AB; 2Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.; 3Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA., Scott CF; 2Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.; 4Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA., Spraggins JM; 1Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA; email: jeff.spraggins@vanderbilt.edu.; 2Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.; 3Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.; 4Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA.; 5Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA. |
| Abstrakt: |
Imaging mass spectrometry (IMS) enables highly multiplexed, untargeted tissue mapping for a broad range of molecular classes, facilitating in situ biological discovery. Yet, challenges persist in molecular specificity, which is the ability to discern one molecule from another, and spatial specificity, which is the ability to link untargeted imaging data to specific tissue features. Instrumental developments have dramatically improved IMS spatial resolution, allowing molecular observations to be more readily associated with distinct tissue features across spatial scales, ranging from larger anatomical regions to single cells. High-performance mass analyzers and systems integrating ion mobility technologies are also becoming more prevalent, further improving molecular coverage and the ability to discern chemical identity. This review provides an overview of recent advancements in high-specificity IMS that are providing critical biological context to untargeted molecular imaging, enabling integrated analyses, and addressing advanced biomedical research applications. |
