A Cool Look at Positive-Strand RNA Virus Replication Organelles: New Insights from Cryo-Electron Microscopy.
| Autor: | de Beijer NL; Molecular Virology Laboratory, Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Snijder EJ; Molecular Virology Laboratory, Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Bárcena M; Electron Microscopy Section, Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands; email: m.barcena@lumc.nl. |
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| Jazyk: | angličtina |
| Zdroj: | Annual review of biochemistry [Annu Rev Biochem] 2024 Aug; Vol. 93 (1), pp. 163-187. Date of Electronic Publication: 2024 Jul 02. |
| DOI: | 10.1146/annurev-biochem-052521-115736 |
| Abstrakt: | Positive-strand RNA viruses encompass a variety of established and emerging eukaryotic pathogens. Their genome replication is confined to specialized cytoplasmic membrane compartments known as replication organelles (ROs). These ROs derive from host membranes, transformed into distinct structures such as invaginated spherules or intricate membrane networks including single- and/or double-membrane vesicles. ROs play a vital role in orchestrating viral RNA synthesis and evading detection by innate immune sensors of the host. In recent years, groundbreaking cryo-electron microscopy studies conducted with several prototypic viruses have significantly advanced our understanding of RO structure and function. Notably, these studies unveiled the presence of crown-shaped multimeric viral protein complexes that seem to actively participate in viral RNA synthesis and regulate the release of newly synthesized RNA into the cytosol for translation and packaging. These findings have shed light on novel viral functions and fascinating macromolecular complexes that delineate promising new avenues for future research. |
| Databáze: | MEDLINE |
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