Dietary restriction reveals sex-specific expression of the mTOR pathway genes in Japanese quails.

Autor: Reda GK; Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary. gebrek2000@agr.unideb.hu.; Doctoral School of Animal Science, University of Debrecen, 4032, Debrecen, Hungary. gebrek2000@agr.unideb.hu.; Department of Evolutionary Zoology and Human Biology, University of Debrecen, 4032, Debrecen, Hungary. gebrek2000@agr.unideb.hu., Ndunguru SF; Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary.; Doctoral School of Animal Science, University of Debrecen, 4032, Debrecen, Hungary.; Department of Evolutionary Zoology and Human Biology, University of Debrecen, 4032, Debrecen, Hungary., Csernus B; Department of Evolutionary Zoology and Human Biology, University of Debrecen, 4032, Debrecen, Hungary., Knop R; Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary., Lugata JK; Doctoral School of Animal Science, University of Debrecen, 4032, Debrecen, Hungary.; Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032, Debrecen, Hungary., Szabó C; Department of Animal Nutrition and Physiology, Faculty of Agriculture and Food Sciences and Environmental Management, University of Debrecen, 4032, Debrecen, Hungary., Czeglédi L; Department of Animal Science, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, University of Debrecen, 4032, Debrecen, Hungary., Lendvai ÁZ; Department of Evolutionary Zoology and Human Biology, University of Debrecen, 4032, Debrecen, Hungary.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 09; Vol. 14 (1), pp. 8314. Date of Electronic Publication: 2024 Apr 09.
DOI: 10.1038/s41598-024-58487-9
Abstrakt: Limited resources affect an organism's physiology through the conserved metabolic pathway, the mechanistic target of rapamycin (mTOR). Males and females often react differently to nutritional limitation, but whether it leads to differential mTOR pathway expression remains unknown. Recently, we found that dietary restriction (DR) induced significant changes in the expression of mTOR pathway genes in female Japanese quails (Coturnix japonica). We simultaneously exposed 32 male and female Japanese quails to either 20%, 30%, 40% restriction or ad libitum feeding for 14 days and determined the expression of six key genes of the mTOR pathway in the liver to investigate sex differences in the expression patterns. We found that DR significantly reduced body mass, albeit the effect was milder in males compared to females. We observed sex-specific liver gene expression. DR downregulated mTOR expression more in females than in males. Under moderate DR, ATG9A and RPS6K1 expressions were increased more in males than in females. Like females, body mass in males was correlated positively with mTOR and IGF1, but negatively with ATG9A and RS6K1 expressions. Our findings highlight that sexes may cope with nutritional deficits differently and emphasise the importance of considering sexual differences in studies of dietary restriction.
(© 2024. The Author(s).)
Databáze: MEDLINE
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