| Autor: |
Valero-Rubira I; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain., Vallés MP; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain., Echávarri B; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain., Fustero P; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain., Costar MA; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain., Castillo AM; Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain. |
| Abstrakt: |
The use of doubled haploid (DH) technology enables the development of new varieties of plants in less time than traditional breeding methods. In microspore embryogenesis (ME), stress treatment triggers microspores towards an embryogenic pathway, resulting in the production of DH plants. Epigenetic modifiers have been successfully used to increase ME efficiency in a number of crops. In wheat, only the histone deacetylase inhibitor trichostatin A (TSA) has been shown to be effective. In this study, inhibitors of epigenetic modifiers acting on histone methylation (chaetocin and CARM1 inhibitor) and histone phosphorylation (aurora kinase inhibitor II (AUKI-II) and hesperadin) were screened to determine their potential in ME induction in high- and mid-low-responding cultivars. The use of chaetocin and AUKI-II resulted in a higher percentage of embryogenic structures than controls in both cultivars, but only AUKI-II was superior to TSA. In order to evaluate the potential of AUKI-II in terms of increasing the number of green DH plants, short and long application strategies were tested during the mannitol stress treatment. The application of 0.8 µM AUKI-II during a long stress treatment resulted in a higher percentage of chromosome doubling compared to control DMSO in both cultivars. This concentration produced 33% more green DH plants than the control in the mid-low-responding cultivar, but did not affect the final ME efficiency in a high-responding cultivar. This study has identified new epigenetic modifiers whose use could be promising for increasing the efficiency of other systems that require cellular reprogramming. |
