Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics.

Autor: Futami M; Department of Engineering, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, Graduate School of University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan., Naito H; Department of Engineering, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, Graduate School of University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan., Ninomiya S; Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan., Chen LC; Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan., Iwano T; Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, 409-3898, Japan., Yoshimura K; Division of Molecular Biology, Center for Medical Education and Sciences, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, 409-3898, Japan., Ukita Y; Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan. yukita@yamanashi.ac.jp.
Jazyk: angličtina
Zdroj: Biomedical microdevices [Biomed Microdevices] 2024 Apr 09; Vol. 26 (2), pp. 22. Date of Electronic Publication: 2024 Apr 09.
DOI: 10.1007/s10544-024-00703-4
Abstrakt: We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device effectively removed the protein aggregates from the sample, and the performance was comparable to those of conventional manual sample preparation procedures that use high-speed centrifugation. In addition, probe electrospray ionization mass spectrometry (PESI-MS) was performed to compare the device-treated and manually treated samples. The obtained PESI-MS spectra were analyzed by partial least squares discriminant analysis, and the preparation capability of the device was found to be equivalent to that of the conventional method.
(© 2024. The Author(s).)
Databáze: MEDLINE
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