Identification of microRNAs and their target genes associated with chasmogamous and cleistogamous flower development in Viola prionantha.

Autor: Li Q; Life Science College, Northwest Normal University, Anning East Road 967, Anning, Lanzhou, 730070, Gansu, China. liqiaoxia8024@nwnu.edu.cn., Zhang Z; Life Science College, Northwest Normal University, Anning East Road 967, Anning, Lanzhou, 730070, Gansu, China., Li K; State Key Laboratory of Plant Diversity and Specialty Crops / State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China.; China National Botanical Garden, Beijing, 100093, China.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China., Zhu Y; Life Science College, Northwest Normal University, Anning East Road 967, Anning, Lanzhou, 730070, Gansu, China., Sun K; Life Science College, Northwest Normal University, Anning East Road 967, Anning, Lanzhou, 730070, Gansu, China., He C; State Key Laboratory of Plant Diversity and Specialty Crops / State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China. chaoying@ibcas.ac.cn.; China National Botanical Garden, Beijing, 100093, China. chaoying@ibcas.ac.cn.; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China. chaoying@ibcas.ac.cn.
Jazyk: angličtina
Zdroj: Planta [Planta] 2024 Apr 09; Vol. 259 (5), pp. 116. Date of Electronic Publication: 2024 Apr 09.
DOI: 10.1007/s00425-024-04398-y
Abstrakt: Main Conclusion: Differentially expressed microRNAs were found associated with the development of chasmogamous and cleistogamous flowers in Viola prionantha, revealing potential roles of microRNAs in the developmental evolution of dimorphic flowers. In Viola prionantha, chasmogamous (CH) flowers are induced by short daylight, while cleistogamous (CL) flowers are triggered by long daylight. How environmental factors and microRNAs (miRNAs) affect dimorphic flower formation remains unknown. In this study, small RNA sequencing was performed on CH and CL floral buds at different developmental stages in V. prionantha, differentially expressed miRNAs (DEmiRNAs) were identified, and their target genes were predicted. In CL flowers, Viola prionantha miR393 (vpr-miR393a/b) and vpr-miRN3366 were highly expressed, while in CH flowers, vpr-miRN2005, vpr-miR172e-2, vpr-miR166m-3, vpr-miR396f-2, and vpr-miR482d-2 were highly expressed. In the auxin-activated signaling pathway, vpr-miR393a/b and vpr-miRN2005 could target Vpr-TIR1/AFB and Vpr-ARF2, respectively, and other DEmiRNAs could target genes involved in the regulation of transcription, e.g., Vpr-AP2-7. Moreover, Vpr-UFO and Vpr-YAB5, the main regulators in petal and stamen development, were co-expressed with Vpr-TIR1/AFB and Vpr-ARF2 and showed lower expression in CL flowers than in CH flowers. Some V. prionantha genes relating to the stress/defense responses were co-expressed with Vpr-TIR1/AFB, Vpr-ARF2, and Vpr-AP2-7 and highly expressed in CL flowers. Therefore, in V. prionantha, CH-CL flower development may be regulated by the identified DEmiRNAs and their target genes, thus providing the first insight into the formation of dimorphic flowers in Viola.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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