| Autor: |
Bergamo ETP; Department of Prosthodontics, NYU Dentistry, New York, New York 10010, United States., Witek L; Biomaterials Division, NYU Dentistry, New York, New York 10010, United States.; Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York 11201, United States.; Hansjörg Wyss Department of Plastic Surgery, NYU Grossman School of Medicine, New York, New York 10016, United States., Ramalho IS; Department of Prosthodontics and Periodontology, University of Sao Paulo, Bauru School of Dentistry, Bauru, SP 17012-230, Brazil., Lopes ACO; Department of Prosthodontics and Periodontology, University of Sao Paulo, Bauru School of Dentistry, Bauru, SP 17012-230, Brazil., Nayak VV; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida 33136, United States., Torroni A; Hansjörg Wyss Department of Plastic Surgery, NYU Grossman School of Medicine, New York, New York 10016, United States., Slavin BV; University of Miami Miller School of Medicine, Miami, Florida 33136, United States., Bonfante EA; Department of Prosthodontics and Periodontology, University of Sao Paulo, Bauru School of Dentistry, Bauru, SP 17012-230, Brazil., Uhrich KE; Department of Chemistry, University of California Riverside, Riverside, California 92521, United States., Graves DT; Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States., Coelho PG; Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida 33136, United States.; Division of Plastic Surgery, Department of Surgery, University of Miami Miller School of Medicine, Miami, Florida 33136, United States. |
| Abstrakt: |
To develop a peri-implantitis model in a Gottingen minipig and evaluate the effect of local application of salicylic acid poly(anhydride-ester) (SAPAE) on peri-implantitis progression in healthy, metabolic syndrome (MS), and type-2 diabetes mellitus (T2DM) subjects. Eighteen animals were allocated to three groups: (i) control, (ii) MS (diet for obesity induction), and (iii) T2DM (diet plus streptozotocin for T2DM induction). Maxillary and mandible premolars and first molar were extracted. After 3 months of healing, four implants per side were placed in both jaws of each animal. After 2 months, peri-implantitis was induced by plaque formation using silk ligatures. SAPAE polymer was mixed with mineral oil (3.75 mg/μL) and topically applied biweekly for up to 60 days to halt peri-implantitis progression. Periodontal probing was used to assess pocket depth over time, followed by histomorphologic analysis of harvested samples. The adopted protocol resulted in the onset of peri-implantitis, with healthy minipigs taking twice as long to reach the same level of probing depth relative to MS and T2DM subjects (∼3.0 mm), irrespective of jaw. In a qualitative analysis, SAPAE therapy revealed decreased levels of inflammation in the normoglycemic, MS, and T2DM groups. SAPAE application around implants significantly reduced the progression of peri-implantitis after ∼15 days of therapy, with ∼30% lower probing depth for all systemic conditions and similar rates of probing depth increase per week between the control and SAPAE groups. MS and T2DM conditions presented a faster progression of the peri-implant pocket depth. SAPAE treatment reduced peri-implantitis progression in healthy, MS, and T2DM groups. |
