Viruses associated with measles-like illnesses in Uganda.

Autor: Namuwulya P; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Ashraf S; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Niebel M; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Ssekagiri A; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Tushabe P; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Kakooza P; Ministry of Health, Kampala, Uganda., Tong L; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Bukenya H; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Jerome H; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Davis C; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Birungi M; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Turyahabwe I; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Mugaga A; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Eliku JP; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Francis A; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Nakabazzi L; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Nsubuga F; Ministry of Health, Kampala, Uganda., Katushabe E; World Health Organization, Kampala, Uganda., Kisakye A; World Health Organization, Kampala, Uganda., Ampeire I; Ministry of Health, Kampala, Uganda., Nanteza A; College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda., Kaleebu P; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Bakamutumaho B; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Nsamba P; College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda., Kazibwe A; College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda., da Silva Filipe A; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK., Tweyongyere R; College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda., Bwogi J; Uganda Virus Research Institute (UVRI), Entebbe, Uganda., Thomson EC; MRC - University of Glasgow Centre for Virus Research (CVR), Glasgow, UK; London School of Hygiene and Tropical Medicine (LSHTM), London, UK. Electronic address: Emma.Thomson@glasgow.ac.uk.
Jazyk: angličtina
Zdroj: The Journal of infection [J Infect] 2024 May; Vol. 88 (5), pp. 106148. Date of Electronic Publication: 2024 Apr 06.
DOI: 10.1016/j.jinf.2024.106148
Abstrakt: Objectives: In this study, we investigated the causes of measles-like illnesses (MLI) in the Uganda national surveillance program in order to inform diagnostic assay selection and vaccination strategies.
Methods: We used metagenomic next-generation sequencing (M-NGS) on the Illumina platform to identify viruses associated with MLI (defined as fever and rash in the presence of either cough, coryza or conjunctivitis) in patient samples that had tested IgM negative for measles between 2010 and 2019.
Results: Viral genomes were identified in 87/271 (32%) of samples, of which 44/271 (16%) contained 12 known viral pathogens. Expected viruses included rubella, human parvovirus B19, Epstein Barr virus, human herpesvirus 6B, human cytomegalovirus, varicella zoster virus and measles virus (detected within the seronegative window-period of infection) and the blood-borne hepatitis B virus. We also detected Saffold virus, human parvovirus type 4, the human adenovirus C2 and vaccine-associated poliovirus type 1.
Conclusions: The study highlights the presence of undiagnosed viruses causing MLI in Uganda, including vaccine-preventable illnesses. NGS can be used to monitor common viral infections at a population level, especially in regions where such infections are prevalent, including low and middle income countries to guide vaccination policy and optimize diagnostic assays.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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