Genetic mechanism regulating diversity in the placement of eyes on the head of animals.

Autor:	Puli OR; Department of Biology, University of Dayton, Dayton, OH 45469., Gogia N; Department of Biology, University of Dayton, Dayton, OH 45469., Chimata AV; Department of Biology, University of Dayton, Dayton, OH 45469., Yorimitsu T; Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan., Nakagoshi H; Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan., Kango-Singh M; Department of Biology, University of Dayton, Dayton, OH 45469.; Premedical Program, University of Dayton, Dayton, OH 45469.; Integrative Science and Engineering, University of Dayton, Dayton, OH 45469., Singh A; Department of Biology, University of Dayton, Dayton, OH 45469.; Premedical Program, University of Dayton, Dayton, OH 45469.; Integrative Science and Engineering, University of Dayton, Dayton, OH 45469.; Center for Genomic Advocacy (TCGA), Indiana State University, Terre Haute, IN 47809.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Apr 16; Vol. 121 (16), pp. e2316244121. Date of Electronic Publication: 2024 Apr 08.
DOI:	10.1073/pnas.2316244121
Abstrakt:	Despite the conservation of genetic machinery involved in eye development, there is a strong diversity in the placement of eyes on the head of animals. Morphogen gradients of signaling molecules are vital to patterning cues. During Drosophila eye development, Wingless (Wg), a ligand of Wnt/Wg signaling, is expressed anterolaterally to form a morphogen gradient to determine the eye- versus head-specific cell fate. The underlying mechanisms that regulate this process are yet to be fully understood. We characterized defective proventriculus (dve) ( Drosophila ortholog of human SATB1), a K50 homeodomain transcription factor, as a dorsal eye gene, which regulates Wg signaling to determine eye versus head fate. Across Drosophila species, Dve is expressed in the dorsal head vertex region where it regulates wg transcription. Second, Dve suppresses eye fate by down-regulating retinal determination genes. Third, the dve -expressing dorsal head vertex region is important for Wg-mediated inhibition of retinal cell fate, as eliminating the Dve-expressing cells or preventing Wg transport from these dve -expressing cells leads to a dramatic expansion of the eye field. Together, these findings suggest that Dve regulates Wg expression in the dorsal head vertex, which is critical for determining eye versus head fate. Gain-of-function of SATB1 exhibits an eye fate suppression phenotype similar to Dve. Our data demonstrate a conserved role for Dve/SATB1 in the positioning of eyes on the head and the interocular distance by regulating Wg. This study provides evidence that dysregulation of the Wg morphogen gradient results in developmental defects such as hypertelorism in humans where disproportionate interocular distance and facial anomalies are reported. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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