Transcriptomic atlas of midbrain dopamine neurons uncovers differential vulnerability in a Parkinsonism lesion model.

Autor:	Yaghmaeian Salmani B; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden., Lahti L; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden., Gillberg L; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden., Jacobsen JK; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.; Department of Neurology, Karolinska University Hospital, Stockholm, Sweden., Mantas I; Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden., Svenningsson P; Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden., Perlmann T; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Apr 08; Vol. 12. Date of Electronic Publication: 2024 Apr 08.
DOI:	10.7554/eLife.89482
Abstrakt:	Midbrain dopamine (mDA) neurons comprise diverse cells with unique innervation targets and functions. This is illustrated by the selective sensitivity of mDA neurons of the substantia nigra compacta (SNc) in patients with Parkinson's disease, while those in the ventral tegmental area (VTA) are relatively spared. Here, we used single nuclei RNA sequencing (snRNA-seq) of approximately 70,000 mouse midbrain cells to build a high-resolution atlas of mouse mDA neuron diversity at the molecular level. The results showed that differences between mDA neuron groups could best be understood as a continuum without sharp differences between subtypes. Thus, we assigned mDA neurons to several 'territories' and 'neighborhoods' within a shifting gene expression landscape where boundaries are gradual rather than discrete. Based on the enriched gene expression patterns of these territories and neighborhoods, we were able to localize them in the adult mouse midbrain. Moreover, because the underlying mechanisms for the variable sensitivities of diverse mDA neurons to pathological insults are not well understood, we analyzed surviving neurons after partial 6-hydroxydopamine (6-OHDA) lesions to unravel gene expression patterns that correlate with mDA neuron vulnerability and resilience. Together, this atlas provides a basis for further studies on the neurophysiological role of mDA neurons in health and disease. Competing Interests: BY, LL, LG, JJ, IM, PS, TP No competing interests declared (© 2023, Yaghmaeian Salmani et al.)
Databáze:	MEDLINE
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