Tunable In Situ Synthesis of Ultrathin Extracellular Matrix-Derived Membranes in Organ-on-a-Chip Devices.

Autor: Newton JD; Department of Mechanical & Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, ON, M5S 3G8, Canada., Song Y; Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 656 Bay Street, Toronto, ON, M5G 0A4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Road, Toronto, ON, M5S 1A8, Canada., Park S; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada., Kanagarajah KR; Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 656 Bay Street, Toronto, ON, M5G 0A4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Road, Toronto, ON, M5S 1A8, Canada., Wong AP; Program in Developmental and Stem Cell Biology, Hospital for Sick Children, 656 Bay Street, Toronto, ON, M5G 0A4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Road, Toronto, ON, M5S 1A8, Canada., Young EWK; Department of Mechanical & Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, ON, M5S 3G8, Canada.; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Aug; Vol. 13 (20), pp. e2401158. Date of Electronic Publication: 2024 Apr 19.
DOI: 10.1002/adhm.202401158
Abstrakt: Thin cell culture membranes in organ-on-a-chip (OOC) devices are used to model a wide range of thin tissues. While early and most current platforms use microporous or fibrous elastomeric or thermoplastic membranes, there is an emerging class of devices using extra-cellular matrix (ECM) protein-based membranes to improve their biological relevance. These ECM-based membranes present physiologically relevant properties, but they are difficult to integrate into OOC devices due to their relative fragility. Additionally, the specialized fabrication methods developed to date make comparison between methods difficult. This work presents the development and characterization of a method to produce ultrathin matrix-derived membranes (UMM) in OOC devices that requires only a preassembled thermoplastic device and a micropipette, decoupling the device and UMM fabrication processes. Control over the thickness and permeability of the UMM is demonstrated, along with integration of the UMM in a device enabling high-resolution on-chip microscopy. The reliability of the UMM fabrication method is leveraged to develop a medium-throughput well-plate format device with 32 independent UMM-integrated samples. Finally, proof-of-concept cell culture experiments are demonstrated. Due to its simplicity and controllability, the presented method has the potential to overcome technical barriers preventing wider adoption of physiologically relevant ECM-based membranes in OOC devices.
(© 2024 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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