Augmin complex activity finetunes dendrite morphology through non-centrosomal microtubule nucleation in vivo.

Autor:	Zhang Y; German Center for Neurodegenerative Diseases (DZNE), Dynamics of Neuronal Circuits Group, Venusberg Campus 1 Building 99, 53127 Bonn, Germany., Sung HH; Institute of Molecular Biology, Academia Sinica, 11529 Taipei, Taiwan., Ziegler AB; German Center for Neurodegenerative Diseases (DZNE), Dynamics of Neuronal Circuits Group, Venusberg Campus 1 Building 99, 53127 Bonn, Germany., Wu YC; Institute of Molecular Biology, Academia Sinica, 11529 Taipei, Taiwan., Viais R; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10, 08028 Barcelona, Spain., Sánchez-Huertas C; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10, 08028 Barcelona, Spain., Kilo L; German Center for Neurodegenerative Diseases (DZNE), Dynamics of Neuronal Circuits Group, Venusberg Campus 1 Building 99, 53127 Bonn, Germany., Agircan FG; German Center for Neurodegenerative Diseases (DZNE), Dynamics of Neuronal Circuits Group, Venusberg Campus 1 Building 99, 53127 Bonn, Germany., Cheng YJ; Institute of Molecular Biology, Academia Sinica, 11529 Taipei, Taiwan., Mouri K; Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan., Uemura T; Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.; Center for Living Systems Information Science, Kyoto University., Lüders J; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac 10, 08028 Barcelona, Spain., Chien CT; Institute of Molecular Biology, Academia Sinica, 11529 Taipei, Taiwan., Tavosanis G; German Center for Neurodegenerative Diseases (DZNE), Dynamics of Neuronal Circuits Group, Venusberg Campus 1 Building 99, 53127 Bonn, Germany.; LIMES Institute, University of Bonn, 53115 Bonn, Germany.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2024 May 01; Vol. 137 (9). Date of Electronic Publication: 2024 May 10.
DOI:	10.1242/jcs.261512
Abstrakt:	During development, neurons achieve a stereotyped neuron type-specific morphology, which relies on dynamic support by microtubules (MTs). An important player is the augmin complex (hereafter augmin), which binds to existing MT filaments and recruits the γ-tubulin ring complex (γ-TuRC), to form branched MTs. In cultured neurons, augmin is important for neurite formation. However, little is known about the role of augmin during neurite formation in vivo. Here, we have revisited the role of mammalian augmin in culture and then turned towards the class four Drosophila dendritic arborization (c4da) neurons. We show that MT density is maintained through augmin in cooperation with the γ-TuRC in vivo. Mutant c4da neurons show a reduction of newly emerging higher-order dendritic branches and in turn also a reduced number of their characteristic space-filling higher-order branchlets. Taken together, our data reveal a cooperative function for augmin with the γ-TuRC in forming enough MTs needed for the appropriate differentiation of morphologically complex dendrites in vivo. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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