Quantitative proteomics analysis reveals an important role of the transcriptional regulator UidR in the bacterial biofilm formation of Aeromonas hydrophila .
Autor: | Li X; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; School of Life Sciences, Nanjing Agricultural University, Nanjing, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China., Tian F; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Zhang B; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Institute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, China., Zhang L; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Chen X; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Lin X; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Wang Y; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Lin X; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.; Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China., Liu Y; College of Life Sciences, College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.; National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, China. |
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Jazyk: | angličtina |
Zdroj: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Mar 22; Vol. 14, pp. 1380747. Date of Electronic Publication: 2024 Mar 22 (Print Publication: 2024). |
DOI: | 10.3389/fcimb.2024.1380747 |
Abstrakt: | Introduction: Bacterial biofilm is a well-known characteristic that plays important roles in diverse physiological functions, whereas the current intrinsic regulatory mechanism of its formation is still largely unknown. Methods: In the present study, a label-free based quantitative proteomics technology was conducted to compare the differentially expressed proteins (DEPs) between ΔuidR and the wild-type strain in the biofilm state. Results: The results showed that the deletion of gene uidR encoding a TetR transcriptional regulator significantly increased the biofilm formation in Aeromonas hydrophila . And there was a total of 220 DEPs, including 120 up-regulated proteins and 100 down-regulated proteins between ΔuidR and the wild-type strain based on the quantitative proteomics. Bioinformatics analysis suggested that uidR may affect bacterial biofilm formation by regulating some related proteins in glyoxylic acid and dicarboxylic acid pathway. The expressions of selected proteins involved in this pathway were further confirmed by q-PCR assay, and the results was in accordance with the quantitative proteomics data. Moreover, the deletion of four genes ( AHA_3063, AHA_3062, AHA_4140 and aceB ) related to the glyoxylic acid and dicarboxylic acid pathway lead to a significant decrease in the biofilm formation. Discussion: Thus, the results indicated that uidR involved in the regulatory of bacterial biofilm formation, and it may provide a potential target for the drug development and a new clue for the prevention of pathogenic A. hydrophila in the future. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Li, Tian, Zhang, Zhang, Chen, Lin, Wang, Lin and Liu.) |
Databáze: | MEDLINE |
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