Oxidative stress induction by essential oil from Piper alatipetiolatum (Piperaceae) triggers lethality in the larvae of Culex quinquefasciatus (Diptera: Culicidae).

Autor: da Costa MLL; Laboratório de Controle Biológico e Biotecnologia da Malária e da Dengue, Coordenação Sociedade, Ambiente e Saúde, Instituto Nacional de Pesquisas da Amazônia,Manaus 69067-375, Amazonas, Brazil. Electronic address: luiza3646@hotmail.com., de Oliveira AC; Laboratório de Controle Biológico e Biotecnologia da Malária e da Dengue, Coordenação Sociedade, Ambiente e Saúde, Instituto Nacional de Pesquisas da Amazônia,Manaus 69067-375, Amazonas, Brazil; Central Analítica, Centro de Apoio Multidisciplinar, Universidade Federal do Amazonas, Manaus 69080-900, Amazonas, Brazil., Roque RA; Laboratório de Controle Biológico e Biotecnologia da Malária e da Dengue, Coordenação Sociedade, Ambiente e Saúde, Instituto Nacional de Pesquisas da Amazônia,Manaus 69067-375, Amazonas, Brazil.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Mar; Vol. 200, pp. 105809. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1016/j.pestbp.2024.105809
Abstrakt: Culex quinquefasciatus is the main vector of lymphatic filariasis in Brazil, which present resistance to commercial insecticides. Nowadays, essential oils (EOs) exhibiting larvicidal activity, such as those derived from Piper alatipetiolatum, provide a promising alternative for vector control, including Culex species. This study aimed to investigate the larvicidal activity and the oxidative stress indicators of the EO from P. alatipetiolatum in Cx. quinquefasciatus larvae. The EO was extracted from P. alatipetiolatum leaves using the hydrodistillation method, resulting in a yield of 7.2 ± 0.1%, analysed by gas chromatography coupled with spectrometry and gas chromatography coupled with flame ionization detector (GC-MS and GC-FID), and evaluated against Cx. quinquefasciatus larvae. Reactive Oxygen and Nitrogen Species (RONS), Catalase (CAT), glutathione-S-transferase (GST), acetylcholinesterase (AChE), and Thiol levels were used as oxidative stress indicators. Analysis by CG-MS and CG-FID revealed that the main compound in the EO was the oxygenated sesquiterpene ishwarone, constituting 78.6% of the composition. Furthermore, the EO exhibited larvicidal activity, ranging from 26 to 100%, with an LC 50 of 4.53 μg/mL and LC 90 of 15.37 μg/mL. This activity was accompanied by a significant increase in RONS production, alterations in CAT, GST, AChE activity, and thiol levels compared to the control groups (p < 0.05). To the best of our knowledge, this is the first report describing the larvicidal activity and oxidative stress induced by the EO from P. alatipetiolatum against Cx. quinquefasciatus larvae. Therefore, we propose that this EO shows promise as larvicidal agent for the effective control of Cx. quinquefasciatus larvae.
Competing Interests: Declaration of competing interest The authors have no relevant financial or non-financial interests to disclose.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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