Synergistic impact of heat and salicylic acid pretreatment on gluten films: Characterization and functional properties.

Autor: Rasheed F; Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: faiza@qau.edu.pk., Saeed MB; Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Fatima S; Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Sajjad A; Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Khan MA; Renewable Energy Advancement Laboratory (REAL), Department of Environmental Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Kayani WK; Department of Biotechnology, University of Kotli, Azad Jammu and Kashmir, Pakistan. Electronic address: wkkayani@uokajk.edu.pk.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 267 (Pt 1), pp. 131402. Date of Electronic Publication: 2024 Apr 04.
DOI: 10.1016/j.ijbiomac.2024.131402
Abstrakt: This study investigates how wheat gluten (WG) films in the presence of salicylic acid are influenced by thermal pretreatment. Unlike previous methods conducted at low moisture content, our procedure involves pretreating WG at different temperatures (65 °C, 75 °C, and 85 °C), in a solution with salicylic acid. This pretreatment aims to enhance protein unfolding, thus providing more opportunities for protein-protein interactions during the subsequent solvent casting into films. A significant increase in β-sheet structures was observed in FTIR spectra of samples pretreated at 75 °C and 85 °C, showing a prominent peak in the range of 1630-1640 cm -1 . The pretreatment at 85 °C was found to be effective in improving the water resistivity of the films by up to 247 %. Moreover, it led to a significant enhancement of 151 % in tensile strength and a 45 % increase in the elastic modulus. The reduced solubility observed in films derived from pretreated WG suggests the development of an intricate protein network arising from protein-protein interactions during the pretreatment and film formation. Thermal pretreatment at 85 °C significantly enhances the structural and mechanical properties of WG films, including improved water resistivity, tensile strength, and intricate protein network formation.
Competing Interests: Declaration of competing interest The authors of the above-mentioned manuscript declare no competing interest.
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Databáze: MEDLINE
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