Evaluation of Lippia scaberrima Sond. and Aspalathus linearis (Burm.f.) R. Dahlgren extracts on human CYP enzymes and gold nanoparticle synthesis: implications for drug metabolism and cytotoxicity.
| Autor: | Kok AM; Department of Plant and Soil Sciences, University of Pretoria, Pretoria, 0002, South Africa.; Research Fellow, South African International Maritime Institute (SAIMI), Nelson Mandela University, Gqeberha, 6019, South Africa., Juvonen R; School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, 70210, Kuopio, Finland., Pasanen M; School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, 70210, Kuopio, Finland., Mandiwana V; Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre. Council for Scientific and Industrial Research, Pretoria, 0001, South Africa., Kalombo ML; Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre. Council for Scientific and Industrial Research, Pretoria, 0001, South Africa., Ray SS; Centre for Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre, Council for Scientific and Industrial Research, Pretoria, 0002, South Africa., Rikhotso-Mbungela R; Centre for Nanostructures and Advanced Materials, DSI-CSIR Nanotechnology Innovation Centre, Council for Scientific and Industrial Research, Pretoria, 0002, South Africa., Lall N; Department of Plant and Soil Sciences, University of Pretoria, Pretoria, 0002, South Africa. namrita.lall@up.ac.za.; School of Natural Resources, University of Missouri, Columbia, MO, USA. namrita.lall@up.ac.za.; College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India. namrita.lall@up.ac.za.; Senior Research Fellow, Bio-Tech R&D Institute, University of the West Indies, Kingston, Jamaica. namrita.lall@up.ac.za. |
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| Jazyk: | angličtina |
| Zdroj: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2024 Apr 05; Vol. 24 (1), pp. 152. Date of Electronic Publication: 2024 Apr 05. |
| DOI: | 10.1186/s12906-024-04439-9 |
| Abstrakt: | Background: Metabolism is an important component of the kinetic characteristics of herbal constituents, and it often determines the internal dose and concentration of these effective constituents at the target site. The metabolic profile of plant extracts and pure compounds need to be determined for any possible herb-drug metabolic interactions that might occur. Methods: Various concentrations of the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with fermented and unfermented Aspalathus linearis extract were used to determine the inhibitory potential on placental, microsomal and recombinant human hepatic Cytochrome P450 enzymes. Furthermore, the study investigated the synthesis and characterization of gold nanoparticles from the ethanolic extract of Lippia scaberrima as a lead sample. Confirmation and characterization of the synthesized gold nanoparticles were conducted through various methods. Additionally, the cytotoxic properties of the ethanolic extract of Lippia scaberrima were compared with the gold nanoparticles synthesized from Lippia scaberrima using gum arabic as a capping agent. Results: All the samples showed varying levels of CYP inhibition. The most potent inhibition took place for CYP2C19 and CYP1B1 with 50% inhibitory concentration (IC Conclusion: Therefore, the essential oil of Lippia scaberrima, the ethanolic extract of Lippia scaberrima alone and their combinations with Aspalathus linearis do not possess any clinically significant CYP interaction potential and may be further investigated for their adjuvant potential for use in the tuberculosis treatment regimen. Furthermore, it was shown that the cytotoxic potential of the Lippia scaberrima gold nanoparticles was reduced by twofold when compared to the ethanolic extract of Lippia scaberrima. (© 2024. The Author(s).) |
| Databáze: | MEDLINE |
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