Sensitive electrochemical flow injection analysis of H 2 O 2 released from cells with a pass-through mode.

Autor: Chen ML; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China., Qian P; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China., Xia TY; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China., Yu CM; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China., Wu ZQ; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China. Electronic address: zqwu@ntu.edu.cn., Bao N; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China. Electronic address: ningbao@ntu.edu.cn., Huo XL; School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China. Electronic address: hxl362349@ntu.edu.cn.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 May 08; Vol. 1302, pp. 342516. Date of Electronic Publication: 2024 Mar 21.
DOI: 10.1016/j.aca.2024.342516
Abstrakt: Conventional plate electrodes were commonly used in electrochemical flow injection analysis and only part of molecules diffused to the plane of electrodes could be detected, which would limit the performance of electrochemical detection. In this study, a low-cost native stainless steel wire mesh (SSWM) electrode was integrated into a 3D-printed device for electrochemical flow injection analysis with a pass-through mode, which is different compared with previous flow-through mode. This strategy was applied for sensitive analysis of hydrogen peroxide (H 2 O 2 ) released from cells. Under the optimal conditions (the applied potentials, the flow rate and the sample volume), the device exhibits high sensitivity toward H 2 O 2 . Linear relationships could be achieved between electrochemical responses and the concentration of H 2 O 2 ranging from 1 nM to 1 mM. The excellent analytical performance of the SSWM-based device could be attributed to the pass-through mode based on the mesh microstructure and intrinsic catalytic properties for H 2 O 2 by stainless steel. This approach could be further successfully extended for screening of H 2 O 2 released from HeLa cells with electrochemical responses linear to the number of cells in a range of 3 - 1.35 × 10 4  cells with an injection volume of 30 μL. This study revealed the potential of mesh electrodes in electrochemical flow injection analysis for cellular function and pathology and its possible extension in cell counting and on-line analysis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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