Insights from genomic analysis of a novel Coxiella burnetii strain isolated in Israel.

Autor:	Cohen-Gihon I; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Israeli O; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Bilinsky G; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Vasker B; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel., Lazar S; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Beth-Din A; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Zvi A; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Ghanem-Zoubi N; Infectious Diseases Institute, Rambam Health Care Campus, Haifa, Israel., Atiya-Nasagi Y; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.
Jazyk:	angličtina
Zdroj:	New microbes and new infections [New Microbes New Infect] 2024 Mar 26; Vol. 59, pp. 101242. Date of Electronic Publication: 2024 Mar 26 (Print Publication: 2024).
DOI:	10.1016/j.nmni.2024.101242
Abstrakt:	The diagnosis of Q fever is challenging due to nonspecific symptoms and negative standard blood culture results. Serological testing through immunofluorescence assay (IFA) is the most commonly used method for diagnosing this disease. Polymerase chain reaction (PCR) tests can also be used to detect bacterial DNA if taken at an appropriate time. Once the presence of bacteria is confirmed in a sample, an enrichment step is required before characterizing it through sequencing. Cultivating C. burnetii is challenging as it can only be isolated by inoculation into cell culture, embryonated eggs, or animals. In this article, we describe the isolation of C. burnetii from a valve specimen in Vero cells. We conducted genome sequencing and taxonomy profiling of this isolate and were able to determine its taxonomic affiliation. Furthermore, Multispacer sequence typing (MST) analysis suggests that the infection originated from a local strain of C. burnetii found around northern Israel and Lebanon. This novel strain belongs to a previously described genotype MST6, harboring the QpRS plasmid, never reported in Israel. Competing Interests: None declared (© 2024 The Authors.)
Databáze:	MEDLINE
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