| Autor: |
Lopes AR; Centro Interdisciplinar de Pesquisa em Biotecnologia - CIPBiotec, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, Rio Grande do Sul, Brazil.; Programa de Pós-Graduação em Ciências Fisiológicas - Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil., Costa Silva DG; Programa de Pós-Graduação em Ciências Fisiológicas - Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil., Rodrigues NR; Grupo de Pesquisa em Bioquímica e Toxicologia Compostos Bioativos - GBToxBio, Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, Rio Grande do Sul, Brazil., Kemmerich Martins I; Centro Interdisciplinar de Pesquisa em Biotecnologia - CIPBiotec, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, Rio Grande do Sul, Brazil., Paganotto Leandro L; Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica - PPGBTox, Universidade Federal de Santa Maria - UFSM, Santa Maria, Rio Grande do Sul, Brazil., Nunes MEM; Centro Interdisciplinar de Pesquisa em Biotecnologia - CIPBiotec, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, Rio Grande do Sul, Brazil., Posser T; Centro Interdisciplinar de Pesquisa em Biotecnologia - CIPBiotec, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, Rio Grande do Sul, Brazil., Franco J; Centro Interdisciplinar de Pesquisa em Biotecnologia - CIPBiotec, Universidade Federal do Pampa - UNIPAMPA, São Gabriel, Rio Grande do Sul, Brazil. |
| Abstrakt: |
Glutamate is one of the predominant excitatory neurotransmitters released from the central nervous system; however, at high concentrations, this substance may induce excitotoxicity. This phenomenon is involved in numerous neuropathologies. At present, clinically available pharmacotherapeutic agents to counteract glutamatergic excitotoxicity are not completely effective; therefore, research to develop novel compounds is necessary. In this study, the main objective was to determine the pharmacotherapeutic potential of the hydroalcoholic extract of Psidium guajava (PG) in a model of oxidative stress-induced by exposure to glutamate utilizing Danio rerio larvae (zebrafish) as a model. Data showed that treatment with glutamate produced a significant increase in oxidative stress, chromatin damage, apoptosis, and locomotor dysfunction. All these effects were attenuated by pre-treatment with the classical antioxidant N-acetylcysteine (NAC). Treatment with PG inhibited oxidative stress responsible for cellular damage induced by glutamate. However, exposure to PG failed to prevent glutamate-initiated locomotor damage. Our findings suggest that under conditions of oxidative stress, PG can be considered as a promising candidate for treatment of glutamatergic excitotoxicity and consequent neurodegenerative diseases. |
