The adipokines progranulin and omentin - novel regulators of basic ovarian cell functions.

Autor: Sirotkin AV; Faculty of Natural Sciences, Constantine the Philosopher University, Tr. A. Hlinku 1, Nitra, 949 74, Slovakia. asirotkin@ukf.sk., Fabová Z; Faculty of Natural Sciences, Constantine the Philosopher University, Tr. A. Hlinku 1, Nitra, 949 74, Slovakia., Loncová B; Faculty of Natural Sciences, Constantine the Philosopher University, Tr. A. Hlinku 1, Nitra, 949 74, Slovakia., Bauerová M; Faculty of Natural Sciences, Constantine the Philosopher University, Tr. A. Hlinku 1, Nitra, 949 74, Slovakia., Halim Harrath A; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Jazyk: angličtina
Zdroj: Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2024 Apr 04; Vol. 22 (1), pp. 38. Date of Electronic Publication: 2024 Apr 04.
DOI: 10.1186/s12958-024-01215-9
Abstrakt: The present study aimed to examine the effects of progranulin and omentin on basic ovarian cell functions. For this purpose, we investigated the effects of the addition of progranulin and omentin (0, 0.1, 1, or 10 ng/ml) on the viability, proliferation, apoptosis and steroidogenesis of cultured rabbit ovarian granulosa cells. To determine the importance of the interrelationships between granulosa cells and theca cells, we compared the influence of progranulin and omentin on progesterone and estradiol release in cultured granulosa cells and ovarian fragments containing both granulosa cells and theca cells. Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, cell death detection, and ELISA. Both progranulin and omentin increased granulosa cell viability and proliferation and decreased apoptosis. Progranulin increased progesterone release by granulosa cells but reduced progesterone output by ovarian fragments. Progranulin decreased estradiol release by granulosa cells but increased it in ovarian fragments. Omentin reduced progesterone release in both models. Omentin reduced estradiol release by granulosa cells but promoted this release in ovarian fragments. The present observations are the first to demonstrate that progranulin and omentin can be direct regulators of basic ovarian cell functions. Furthermore, the differences in the effects of these adipokines on steroidogenesis via granulosa and ovarian fragments indicate that these peptides could target both granulosa and theca cells.
(© 2024. The Author(s).)
Databáze: MEDLINE
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