Biochemistry and transcriptomic analyses of Phthorimaea absoluta (Lepidoptera: Gelechiidae) response to insecticides.

Autor: Karanu SW; International Centre of Insect Physiology and Ecology, Nairobi, Kenya.; Department of Biochemistry, Egerton University, Egerton, Kenya., Ajene IJ; International Centre of Insect Physiology and Ecology, Nairobi, Kenya., Lelmen EK; Department of Biochemistry, Egerton University, Egerton, Kenya., Ong'onge MA; International Centre of Insect Physiology and Ecology, Nairobi, Kenya., Akutse KS; International Centre of Insect Physiology and Ecology, Nairobi, Kenya.; Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520, South Africa., Khamis FM; International Centre of Insect Physiology and Ecology, Nairobi, Kenya. fkhamis@icipe.org.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 04; Vol. 14 (1), pp. 7931. Date of Electronic Publication: 2024 Apr 04.
DOI: 10.1038/s41598-024-58413-z
Abstrakt: Phthorimaea absoluta is an invasive solanaceous plant pest with highly devastating effects on tomato plant. Heavy reliance on insecticide use to tackle the pest has been linked to insecticide resistance selection in P. absoluta populations. To underline insights on P. absoluta insecticide resistance mechanisms to diamides and avermectins, we evaluated the transcriptomic profile of parental (field-collected) and F8 (lab-reared) populations. Furthermore, to screen for the presence of organophosphate and pyrethroid resistance, we assessed the gene expression levels of acetylcholinesterase (ace1) and para-type voltage-gated sodium channel (VGSG) genes in the F1 to F8 lab-reared progeny of diamide and avermectin exposed P. absoluta field-collected populations. The VGSG gene showed up-regulation in 12.5% and down-regulation in 87.5% of the screened populations, while ace1 gene showed up-regulation in 37.5% and down-regulation in 62.5% of the screened populations. Gene ontology of the differentially expressed genes from both parental and eighth generations of diamide-sprayed P. absoluta populations revealed three genes involved in the metabolic detoxification of diamides in P. absoluta. Therefore, our study showed that the detoxification enzymes found could be responsible for P. absoluta diamide-based resistance, while behavioural resistance, which is stimulus-dependent, could be attributed to P. absoluta avermectin resistance.
(© 2024. The Author(s).)
Databáze: MEDLINE
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