Hypothalamic TRPM8 and TRPA1 ion channel genes in the regulation of temperature homeostasis at water balance changes.

Autor: Kozyreva TV; Institute of Neuroscience and Medicine, Timakov str. 4, Novosibirsk 630117, Russia; Novosibirsk State University, Pirogov str. 2, Novosibirsk 630090, Russia. Electronic address: kozyrevatv@neuronm.ru., Orlov IV; Institute of Neuroscience and Medicine, Timakov str. 4, Novosibirsk 630117, Russia; Novosibirsk State University, Pirogov str. 2, Novosibirsk 630090, Russia. Electronic address: igor-orlow-2199@mail.ru., Boyarskaya AR; Institute of Neuroscience and Medicine, Timakov str. 4, Novosibirsk 630117, Russia. Electronic address: boyarskaya.a.r@gmail.com., Voronova IP; Institute of Neuroscience and Medicine, Timakov str. 4, Novosibirsk 630117, Russia. Electronic address: voronovaip@neuronm.ru.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2024 Apr 01; Vol. 828, pp. 137763. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1016/j.neulet.2024.137763
Abstrakt: The role of the hypothalamic cold-sensitive ion channels - transient receptor potential melastatin 8 (TRPM8) and transient receptor potential ankyrin 1 (TRPA1) in homeostatic systems of thermoregulation and water-salt balance - is not clear. The interaction of homeostatic systems of thermoregulation and water-salt balance without additional temperature load did not receive due attention, too. On the models of water-balance disturbance, we tried to elucidate some aspect of these problems. Body temperature (T body ), O 2 consumption, CO 2 excretion, electrical muscle activity (EMA), temperature of tail skin (T tail ), plasma osmolality, as well as gene expression of hypothalamic TRPM8 and TRPA1 have been registered in rats of 3 groups: control; water-deprived (3 days under dry-eating); and hyperhydrated (6 days without dry food, drinking liquid 4 % sucrose). No relationship was observed between plasma osmolality and gene expression of Trpm8 and Trpa1. In water-deprived rats, the constriction of skin vessels, increased fat metabolism by 10 % and increased EMA by 48 % allowed the animals to maintain T body unchanged. The hyperhydrated rats did not develop sufficient mechanisms, and their T body decreased by 0.8 °C. The development of reactions was correlated with the expression of genes of thermosensitive ion channels in the anterior hypothalamus. T tail had a direct correlation with the expression of the Trpm8 gene, whereas EMA directly correlated with the expression of the Trpa1 gene in water-deprived group. The obtained data attract attention from the point of view of management and correction of physiological functions by modulating the ion channel gene expression.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: